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Titolo:
Possible role of subunit A of nuclear factor Y (NF-YA) in normal human diploid fibroblasts during senescence
Autore:
Matuoka, K; Chen, KY;
Indirizzi:
Dept Chem, Grad Program Mol Biosci, Piscataway, NJ 08854 USA Dept Chem Piscataway NJ USA 08854 am Mol Biosci, Piscataway, NJ 08854 USA Rutgers State Univ, Dept Chem, Piscataway, NJ 08854 USA Rutgers State Univ Piscataway NJ USA 08854 Chem, Piscataway, NJ 08854 USA
Titolo Testata:
BIOGERONTOLOGY
fascicolo: 3, volume: 1, anno: 2000,
pagine: 261 - 271
SICI:
1389-5729(2000)1:3<261:PROSAO>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
CCAAT-BINDING-PROTEIN; THYMIDINE KINASE GENE; REGULATED EXPRESSION; TRANSCRIPTION FACTOR; DNA-BINDING; IN-VIVO; PROMOTER; CBP/TK; CELLS; AGE;
Keywords:
CBP/tk; G1/S genes; NF-Y; P/CAF; replicative senescence; thymidine kinase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Matuoka, K Dept Chem, Grad Program Mol Biosci, Piscataway, NJ 08854 USA Dept Chem Piscataway NJ USA 08854 ci, Piscataway, NJ 08854 USA
Citazione:
K. Matuoka e K.Y. Chen, "Possible role of subunit A of nuclear factor Y (NF-YA) in normal human diploid fibroblasts during senescence", BIOGERONTOL, 1(3), 2000, pp. 261-271

Abstract

NF-Y, a heterotrimeric CCAAT binding protein, may have a role in regulating some Gl/S genes whose expressions are attenuated during replicative senescence [Matuoka and Chen (1999) Exp Cell Res 253. 365-371]. The hallmark of replicative senescence is the loss of dividing potential. Hence, attenuation of Gl/S gene expressions may be causally related to aging. To understand how NF-Y is involved in regulating Gl/S genes during replicative senescence, we have examined the expressions of three NF-Y subunit genes in human IMR-90 cells over the entire course of their life-span. The mRNA levels of NF-YA, B, and C did not show any age-dependent change. In contrast, the protein level of NF-YA exhibited a significant and progressive decrease during cell senescence. Cross-linking experiments indicated that NF-Y may interact with proteins such as GCN5 and P/CAF Co-transfection of cells with plasmid encoding NF-YA protein enhanced the expression of reporter gene fused with Gl/S gene promoter that contains NF-Y sites. In contrast, co-transfection with plasmid encoding the dominant negative NF-YA mutant suppressed the expression of the reporter genes. Transient transfection of human cells with dominant negative NF-YA mutant could lead to an increase in neutral beta -galactosidase activity, a marker of cell senescence. These results support the view that NF-Y may have a rule in cell senescence.

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Documento generato il 08/04/20 alle ore 23:56:27