Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
The His gap motif in microbial lipases: A determinant of stereoselectivitytoward triacylglycerols and analogs
Autore:
Pleiss, J; Scheib, H; Schmid, RD;
Indirizzi:
Univ Stuttgart, Inst Tech Biochem, D-70569 Stuttgart, Germany Univ Stuttgart Stuttgart Germany D-70569 hem, D-70569 Stuttgart, Germany
Titolo Testata:
BIOCHIMIE
fascicolo: 11, volume: 82, anno: 2000,
pagine: 1043 - 1052
SICI:
0300-9084(200011)82:11<1043:THGMIM>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
RHIZOPUS-ORYZAE LIPASE; SEMIEMPIRICAL METHODS; STRUCTURAL BASIS; HYDROLYSIS; ACID; STEREOSPECIFICITY; TRIRADYLGLYCEROLS; ESTERIFICATION; OPTIMIZATION; SELECTIVITY;
Keywords:
lipase; stereoselectivity; alcohol binding site; molecular modeling; His gap motif;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Schmid, RD Univ Stuttgart, Inst Tech Biochem, Allmandring 31, D-70569 Stuttgart, Germany Univ Stuttgart Allmandring 31 Stuttgart Germany D-70569 ermany
Citazione:
J. Pleiss et al., "The His gap motif in microbial lipases: A determinant of stereoselectivitytoward triacylglycerols and analogs", BIOCHIMIE, 82(11), 2000, pp. 1043-1052

Abstract

Lipases preferably hydrolyze the sn-1 and sn-3 acyl chain of triacylglycerols and sn-2 substituted analogs. Molecular modeling studies of the stereopreference of microbial lipases from Rhizopus oryzae, Rhizomucor miehei, Candida rugosa, and lipase B from Candida antarctica toward the hydrolysis of triacylglycerols and analogs revealed that sterical interactions occurring between the sn-2 substituent and the His gap affect substrate geometry, which can be monitored by a single torsion angle. This torsion angle correlates to the experimentally determined stereopreference and is, therefore, suitable to predict stereopreference by molecular modeling. For a given microbial lipase, stereopreference can be estimated by measuring the distance between the side chains of the His gap residues: a narrow His gap cleft impliessn-3 stereopreference for air investigated substrates; a medium-sized His gap discriminates by flexibility of the substrates: flexible substrates arehydrolyzed in sn-1, while rigid substrates are hydrolyzed in sn-3. A wide open His gap implies sn-1 stereopreference for all substrates. This rule holds for all investigated microbial wird type lipases and mutants. (C) 2000 Societe francaise de biochimie et biologie moleculaire / Editions scientifiques et medicales Elsevier.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 00:24:02