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Titolo:
Human class V alcohol dehydrogenase (ADH5): A complex transcription unit generates C-terminal multiplicity
Autore:
Stromberg, P; Hoog, JO;
Indirizzi:
Karolinska Inst, Dept Med Biochem & Biophys, SE-17177 Stockholm, Sweden Karolinska Inst Stockholm Sweden SE-17177 ys, SE-17177 Stockholm, Sweden
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 3, volume: 278, anno: 2000,
pagine: 544 - 549
SICI:
0006-291X(20001130)278:3<544:HCVAD(>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA; EUKARYOTES; ENZYME; SUBUNIT; GENE; CDNA; NOMENCLATURE; BINDING; MOUSE; END;
Keywords:
human alcohol dehydrogenase; fetal; tissue; mRNA processing; polyadenylation; alternative splicing;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Hoog, JO Karolinska Inst, Dept Med Biochem & Biophys, SE-17177 Stockholm, Sweden Karolinska Inst Stockholm Sweden SE-17177 177 Stockholm, Sweden
Citazione:
P. Stromberg e J.O. Hoog, "Human class V alcohol dehydrogenase (ADH5): A complex transcription unit generates C-terminal multiplicity", BIOC BIOP R, 278(3), 2000, pp. 544-549

Abstract

The human ADH5 gene was reported to lack. the last exon compared to other mammalian ADHs and consequently should be expressed as a truncated protein. Here we show with PCR amplification of 3'-cDNA ends that the ADH5 gene harbors the "missing" exon. Besides a cDNA identical to the published sequence, we found full-length transcripts that contained additional codons for eight amino acid residues. Northern blot analysis established the full-length variant as the major transcript with the strongest signal from adult liver. Sequence analysis of genomic DNA confirmed that the ADH5 gene displays composite internal/terminal exons, which can be differentially processed; i.e., 3'-end generation is a result of competition between polyadenylation and splicing. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 07:09:00