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Titolo:
An allele-specific polymerase chain reaction method for the determination of the D85Y polymorphism in the human UDP-glucuronosyltransferase 2B15 genein a case-control study of prostate cancer
Autore:
MacLeod, SL; Nowell, S; Plaxco, J; Lang, NP;
Indirizzi:
Univ Arkansas Med Sci, Dept Surg, Little Rock, AR 72205 USA Univ Arkansas Med Sci Little Rock AR USA 72205 Little Rock, AR 72205 USA Cent Arkansas Vet Healthcare Syst, Little Rock, AR USA Cent Arkansas Vet Healthcare Syst Little Rock AR USA Little Rock, AR USA
Titolo Testata:
ANNALS OF SURGICAL ONCOLOGY
fascicolo: 10, volume: 7, anno: 2000,
pagine: 777 - 782
SICI:
1068-9265(200012)7:10<777:AAPCRM>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
STEROID 5-ALPHA-REDUCTASE; EVOLUTIONARY DIVERGENCE; EXPRESSION; RISK; TISSUES; INTRACRINOLOGY; SUPERFAMILY; METABOLISM; DRUGS; CELLS;
Keywords:
prostate cancer; UDP-glucuronosyltransferase; UGT2B15; androgen metabolism;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: MacLeod, SL Univ Arkansas Med Sci, Dept Surg, 4301 W Markham St,Slot 725, Little Rock,AR 72205 USA Univ Arkansas Med Sci 4301 W Markham St,Slot 725 Little Rock AR USA 72205
Citazione:
S.L. MacLeod et al., "An allele-specific polymerase chain reaction method for the determination of the D85Y polymorphism in the human UDP-glucuronosyltransferase 2B15 genein a case-control study of prostate cancer", ANN SURG O, 7(10), 2000, pp. 777-782

Abstract

Background: UDP-glucuronosyltransferase 2B15 (UGT2B15) catalyzes the inactivation of dihydrotestosterone (DHT) by forming the DHT-glucuronide and is expressed in normal and hyperplastic prostate tissue. Alterations in the activity of this enzyme could be a major contributing factor to the bioavailability of androgens in target tissue such as the prostate. Methods: A polymorphism (D-85 to Y-85) has been identified in the UGT2B15 gene(1) that results in a 50% reduction in enzyme activity. Previously, detection of the polymorphic nucleotide has required direct sequencing. We have developed and validated an allele-specific polymerase chain reaction (PCR) assay to identify the polymorphic base pair in the UGT2B15 gene. This assay was used to examine the distribution of the UGT2B15 polymorphism in a small case-control group (64 cases and 64 controls) from a prostate cancer study. Results: The results of this analysis show that prostate cancer patients were significantly more likely to be homozygous for the lower activity D85 UGT2B15 allele than control individuals (41% versus 19%, respectively, odds ratio = 3.0 (95% confidence intervals 1,3-6.5)),Conclusions: These results suggest that individuals who are homozygous forthe lower activity allele may be at increased risk for developing prostatecancer.

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Documento generato il 29/03/20 alle ore 08:13:25