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Titolo:
A high-performance liquid chromatographic method for the determination of 8-oxo-7,8-dihydro-2 '-deoxyguanosine in urine from man and rat
Autore:
Lengger, C; Schoch, G; Topp, H;
Indirizzi:
Univ Witten Herdecke, Res Inst Child Nutr, D-44225 Dortmund, Germany Univ Witten Herdecke Dortmund Germany D-44225 D-44225 Dortmund, Germany Univ Greifswald, Childrens Hosp, D-17487 Greifswald, Germany Univ Greifswald Greifswald Germany D-17487 , D-17487 Greifswald, Germany
Titolo Testata:
ANALYTICAL BIOCHEMISTRY
fascicolo: 1, volume: 287, anno: 2000,
pagine: 65 - 72
SICI:
0003-2697(200012)287:1<65:AHLCMF>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
OXIDATIVE DNA-DAMAGE; ELECTROCHEMICAL DETECTION; BIOLOGICAL-FLUIDS; ASSAY; 8-HYDROXY-2'-DEOXYGUANOSINE; 8-OXOGUANINE; COLUMN; CANCER; HUMANS;
Keywords:
renal excretion of 8-oxo-7,8-dihydro-2 '-deoxyguanosine; high-performance liquid chromatography and electrochemical detection; human and rat urine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Lengger, C Univ Witten Herdecke, Res Inst Child Nutr, D-44225 Dortmund, Germany Univ Witten Herdecke Dortmund Germany D-44225 rtmund, Germany
Citazione:
C. Lengger et al., "A high-performance liquid chromatographic method for the determination of 8-oxo-7,8-dihydro-2 '-deoxyguanosine in urine from man and rat", ANALYT BIOC, 287(1), 2000, pp. 65-72

Abstract

The renally excreted amount of 8-oxo-7,8-dihydro-2'-deoxyguanosine (oxo(8)dG) is a potential marker of oxidative DNA damage by reactive oxygen species. We have developed a high-performance liquid chromatographic (HPLC) method to determine oxo(8)dG; in urine from humans and Wistar rats. First, 300 mul of filtered urine is prefractionated by solid phase extraction (BAKER-BOND SPE C-18 Polar Plus column). Then, the HPLC separation of the fraction containing oxo(8)dG is performed using four HPLC columns (two cation exchange and two Cls columns) in series with an automated column switching technique. Quantification of oxo(8)dG is performed by electrochemical detection (Coulochem II, ESA Inc.). Limit of detection was 0.4 nM oxo(8)dG. Recovery ofoxo(8)dG added respectively in 11 or 8 concentration steps (range, 4-74 or2-23 nM) to a pooled human or rat urine was 104.1 +/- 4.3 or 104.5 +/- 7.7%. Precision of sixfold analysis of a pooled human or rat urine carried outrespectively on the same day was 2.2 or 2.4% relative standard deviation. Normal excretion rates of oxo(8)dG in healthy adult humans (five females, six males; body weight, 70.7 +/- 11 kg) and male Wistar rats (body weight, 309 +/- 13 g) were 281.7 +/- 179.1 and 333.2 +/- 47.4 pmol oxo(8)dG/ day/kg weight, respectively. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 12:38:01