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Titolo:
MESSENGER-RNAS ENCODING CCKB BUT NOT CCKA RECEPTORS ARE EXPRESSED IN HUMAN T-LYMPHOCYTES AND JURKAT LYMPHOBLASTOID-CELLS
Autore:
CUQ P; GROSS A; TERRAZA A; FOURMY D; CLERC P; DORNAND J; MAGOUS R;
Indirizzi:
FAC PHARM MONTPELLIER,BIOCHIM MEMBRANES LAB,15 AVE CHARLES FLAHAULT F-34060 MONTPELLIER FRANCE FAC PHARM MONTPELLIER,BIOCHIM MEMBRANES LAB F-34060 MONTPELLIER FRANCE UNIV MONTPELLIER 2,INSERM U431 F-34095 MONTPELLIER FRANCE CHU RANGUEIL,INST LOUIS BUGNARD,LAB BIOL & PATHOL DIGEST F-31054 TOULOUSE FRANCE
Titolo Testata:
Life sciences
fascicolo: 5, volume: 61, anno: 1997,
pagine: 543 - 555
SICI:
0024-3205(1997)61:5<543:MECBNC>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
BLOOD MONONUCLEAR-CELLS; NECROSIS-FACTOR-ALPHA; CHOLECYSTOKININ RECEPTOR; FUNCTIONAL EXPRESSION; MOLECULAR-CLONING; CHROMOSOMAL LOCALIZATION; HUMAN BRAIN; LINE; ACTIVATION; CALCIUM;
Keywords:
GASTRIN/CCK RECEPTOR; LYMPHOID JURKAT CELLS; RT-PCR ANALYSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
P. Cuq et al., "MESSENGER-RNAS ENCODING CCKB BUT NOT CCKA RECEPTORS ARE EXPRESSED IN HUMAN T-LYMPHOCYTES AND JURKAT LYMPHOBLASTOID-CELLS", Life sciences, 61(5), 1997, pp. 543-555

Abstract

We previously reported the existence of pharmacologically related gastrin/CCKB type receptors (CCKB-R) in a variant of Jurkat T lymphoblastoid cells (JK(CD3(-)CD4(+))). We studied here the expression of mRNAs encoding CCKA and CCKB receptors in various human white cells by meansof Reverse Transcription-Polymerase Chain Reaction (RT-PCR). Using CCKB-R specific primers, we detected a significant expression of CCKB-R mRNA in JK(CD3(-)CD4(+)) cells. These transcripts were also expressed,at a lower level, in two other Jurkat crones (JK(CD3(+)CD4(-)) and JK(CD3(+)CD4(+))), in peripheral blood lymphocytes (PBL) and in purifiedCD4(+) and CD8(+) lymphocytes. Activation of Jurkat cells and PBL by T cells mitogenic lectins (jacalin, phytohemaglutinin) did not modify CCKB-R mRNA expression. In all these cells, using CCKA-R specific primers, we could not amplify any specific cDNA fragment corresponding to this receptor. Neither CCKB-R nor CCKA-R mRNAs could be detected in monocytic cells. Our data show for the first time a constitutive expression of CCKB-R transcripts in lymphoid cells. Moreover, the modulation of immunocyte functions by cholecystokinin-related peptides could occur through CCKB-R rather than CCKA-R and affect lymphocytes rather thanmonocytes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 19:24:26