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Titolo:
Co-localization of multiple antigens and specific DNA - A novel method using methyl methacrylate-embedded semithin serial sections and catalyzed reporter deposition
Autore:
Mueller, M; Wacker, K; Hickey, WF; Ringelstein, EB; Kiefer, R;
Indirizzi:
Univ Munster, Neurol Klin & Poliklin, Dept Neurol, D-48129 Munster, Germany Univ Munster Munster Germany D-48129 pt Neurol, D-48129 Munster, Germany Dartmouth Med Sch, Dept Pathol, Lebanon, NH USA Dartmouth Med Sch LebanonNH USA h Med Sch, Dept Pathol, Lebanon, NH USA
Titolo Testata:
AMERICAN JOURNAL OF PATHOLOGY
fascicolo: 6, volume: 157, anno: 2000,
pagine: 1829 - 1838
SICI:
0002-9440(200012)157:6<1829:COMAAS>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-SITU HYBRIDIZATION; PEROXIDASE-LABELED OLIGODEOXYNUCLEOTIDES; EXPERIMENTAL AUTOIMMUNE NEURITIS; TYRAMIDE SIGNAL AMPLIFICATION; MARROW-DERIVED ELEMENTS; MESSENGER-RNA DETECTION; INSITU HYBRIDIZATION; NERVOUS-SYSTEM; TRANSFORMING GROWTH-FACTOR-BETA-1; PLASTIC SECTIONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Kiefer, R Univ Munster, Neurol Klin & Poliklin, Dept Neurol, Albert Schweitzer Str 33, D-48129 Munster, Germany Univ Munster Albert Schweitzer Str 33Munster Germany D-48129 y
Citazione:
M. Mueller et al., "Co-localization of multiple antigens and specific DNA - A novel method using methyl methacrylate-embedded semithin serial sections and catalyzed reporter deposition", AM J PATH, 157(6), 2000, pp. 1829-1838

Abstract

Co-localization of proteins and nucleic acid sequences by in situ hybridization and immunohistochemistry is frequently difficult as the process necessary to detect the target structure of one technique may negatively affect the target of the other. Morphological impairment may also limit the application of the two techniques on sensitive tissue. To overcome these problemswe developed a method to perform is situ hybridization and immunohistochemistry on semithin sections of methyl methacrylate-embedded tissue. Microwave-stimulated antigen retrieval, signal amplification by catalyzed reporter deposition, and fluorescent dyes were used for both techniques, yielding high sensitivity and excellent: morphological preservation compared to conventional paraffin sections. Co-localization of in situ hybridization and. immunohistochemistry signals with high morphological resolution was achieved on single sections as well as on adjacent multiple serial sections, using computerized image processing. The latter allowed for the co-localization of multiple antigens and a specific DNA sequence at the same tissue level. Themethod was successfully applied to radiation bone marrow chimeric rats created by transplanting wild-type Lewis rat bone marrow into TK-tsa transgenic Lewis rats, in an attempt to trace and characterize TK-tsa transgenic cells. It also proved useful in the co-localization of multiple antigens in peripheral nerve biopsies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 00:34:41