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Titolo:
Rapid whole blood analysis of virus-specific CD4 and CD8 T cell responses in persistent HIV infection
Autore:
Sester, M; Sester, U; Kohler, H; Schneider, T; Deml, L; Wagner, R; Mueller-Lantzsch, N; Pees, HW; Meyerhans, A;
Indirizzi:
Univ Saarland, Dept Virol, Inst Med Microbiol & Hyg, D-66421 Homburg, Germany Univ Saarland Homburg Germany D-66421 ol & Hyg, D-66421 Homburg, Germany Univ Saarland, Dept Med 4, D-66421 Homburg, Germany Univ Saarland Homburg Germany D-66421 pt Med 4, D-66421 Homburg, Germany Univ Saarland, Dept Med 2, D-66421 Homburg, Germany Univ Saarland Homburg Germany D-66421 pt Med 2, D-66421 Homburg, Germany Univ Saarland, Dept Med 1, D-66421 Homburg, Germany Univ Saarland Homburg Germany D-66421 pt Med 1, D-66421 Homburg, Germany Univ Regensburg, Inst Med Microbiol & Hyg, Dept Virol, D-8400 Regensburg, Germany Univ Regensburg Regensburg Germany D-8400 ol, D-8400 Regensburg, Germany
Titolo Testata:
AIDS
fascicolo: 17, volume: 14, anno: 2000,
pagine: 2653 - 2660
SICI:
0269-9370(200012)14:17<2653:RWBAOV>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
CLASS-I; ANTIRETROVIRAL THERAPY; LYMPHOCYTE ACTIVITY; PEPTIDE COMPLEXES; TYPE-1 INFECTION; IMMUNE-RESPONSES; DENDRITIC CELLS; FLOW-CYTOMETRY; IMMUNODEFICIENCY; ANTIGEN;
Keywords:
antiretroviral therapy; CD4; CD8; cellular immunity; HIV;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Meyerhans, A Univ Saarland, Dept Virol, Inst Med Microbiol & Hyg, Bldg 47,D-66421 Homburg, Germany Univ Saarland Bldg 47 Homburg Germany D-66421 mburg, Germany
Citazione:
M. Sester et al., "Rapid whole blood analysis of virus-specific CD4 and CD8 T cell responses in persistent HIV infection", AIDS, 14(17), 2000, pp. 2653-2660

Abstract

Objectives: Upon HIV infection, strong antiviral cytotoxic and helper T cell responses are generated. They are considered to be an important component in the control of HIV viral load. A simple and rapid whole blood assay was established to quantify and simultaneously characterize HIV-reactive CD4 and CD8 cells. The assay was applied to evaluate the effect of antiretroviral therapy on HIV-specific T cell responses. Methods: Whole blood of 33 HIV-infected individuals was specifically stimulated by HIV-1 pr55(gag), and activation-induced intracellular cytokine expression in CD4 and CD8 T cells was analysed by flow cytometry. Results: HIV-l-specific CD8 and CD4 T cells can be quantified simultaneously. As specific antigen, HIV-1 Pr55(gag) virus-like particles were superiorto soluble protein, especially for the activation of CD8 T cells. In untreated individuals, a high frequency of HIV-specific T cells was observed. The frequency of CD8 T cells was consistently higher than the respective CD4 T cell response, thus demonstrating a dominance in CD8 T cell expansion in persistent HIV infection. Patients on antiretroviral therapy showed a significant reduction in HIV-specific CD4 and, even more strikingly, CD8 T cells. Conclusion: The whole blood assay provides a rapid estimate of the total antiviral T cell resources, and is highly suited for a clinical setting. It may thus have widespread applications for the evaluation of vaccination strategies and immunotherapy. Because antiretroviral therapy significantly reduces both HIV-specific cytotoxic and helper T cell responses, future therapeutic strategies should aim at improving cellular antiviral immunity. (C) 2000 Lippincott Williams & Wilkins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/09/20 alle ore 23:47:08