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Titolo:
Translation initiation of a bicistronic mRNA of borna disease virus: A 16-kDa phosphoprotein is initiated at an internal start codon
Autore:
Kobayashi, T; Watanabe, M; Kamitani, W; Tomonaga, K; Ikuta, K;
Indirizzi:
Osaka Univ, Microbial Dis Res Inst, Dept Virol, Osaka 5650871, Japan OsakaUniv Osaka Japan 5650871 es Inst, Dept Virol, Osaka 5650871, Japan
Titolo Testata:
VIROLOGY
fascicolo: 2, volume: 277, anno: 2000,
pagine: 296 - 305
SICI:
0042-6822(20001125)277:2<296:TIOABM>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
P/C MESSENGER-RNA; INFLUENZA-B VIRUS; BLOOD MONONUCLEAR-CELLS; NUCLEAR-LOCALIZATION; INFECTED-CELLS; P-GENE; GENOMIC ORGANIZATION; RIBOSOMAL INITIATION; STRUCTURAL PROTEINS; READING FRAMES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
64
Recensione:
Indirizzi per estratti:
Indirizzo: Tomonaga, K Osaka Univ, Microbial Dis Res Inst, Dept Virol, 3-1 Yamadaoka,Osaka 5650871, Japan Osaka Univ 3-1 Yamadaoka Osaka Japan 5650871 a 5650871, Japan
Citazione:
T. Kobayashi et al., "Translation initiation of a bicistronic mRNA of borna disease virus: A 16-kDa phosphoprotein is initiated at an internal start codon", VIROLOGY, 277(2), 2000, pp. 296-305

Abstract

We examined translational initiation of a bicistronic 0.8-kb mRNA of Bornadisease virus (BDV) using a cDNA clone of the mRNA. Upon transfection withthe clone, COS-7 cells produced a 16-kDa protein (P'), in addition to the previously identified products of BDV, 24- (P) and 14.5-kDa proteins. The 16-kDa product was detected by anti-P monoclonal antibody and was shown to exist in BDV-infected cell lines as well as in infected animal brain cells. Transient expression analysis of mutated cDNA clones encoding the BDV 0.8-kb mRNA revealed that the 16-kDa protein was initiated at the second AUG codon on the same open reading frame of the P protein. The mutational analysisalso demonstrated that the first AUG within the 0.8-kb mRNA is not optimal, although the signal contains a better Kozak's motif. These results demonstrated the presence of three functional AUG codons in the smallest mRNA of BDV and also suggested that a leaky scanning mechanism is involved in translational initiation at AUG codons downstream of the bicistronic mRNA of BDV. Furthermore, the 16-kDa protein was located in the BDV-specific nuclear foci and was found to associate with the other viral proteins in BDV-infected cells, demonstrating an important role of the novel identified BDV protein in viral replication. (C) 2000 Academic Press.

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Documento generato il 19/01/20 alle ore 08:58:13