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Titolo:
Traffic pattern of cystic fibrosis transmembrane regulator through the early exocytic pathway
Autore:
Bannykh, SI; Bannykh, GI; Fish, KN; Moyer, BD; Riordan, JR; Balch, WE;
Indirizzi:
Scripps Clin & Res Inst, Dept Cell, La Jolla, CA 92037 USA Scripps Clin & Res Inst La Jolla CA USA 92037 ell, La Jolla, CA 92037 USA Scripps Clin & Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA Scripps Clin & Res Inst La Jolla CA USA 92037 iol, La Jolla, CA 92037 USA Inst Childhood & Neglected Dis, La Jolla, CA 92037 USA Inst Childhood & Neglected Dis La Jolla CA USA 92037 Jolla, CA 92037 USA Univ Calif San Diego, Dept Pathol, San Diego, CA 92103 USA Univ Calif San Diego San Diego CA USA 92103 thol, San Diego, CA 92103 USA Mayo Clin & Mayo Fdn, SC Johnson Med Res Ctr, Scottsdale, AZ 85259 USA Mayo Clin & Mayo Fdn Scottsdale AZ USA 85259 tr, Scottsdale, AZ 85259 USA
Titolo Testata:
TRAFFIC
fascicolo: 11, volume: 1, anno: 2000,
pagine: 852 - 870
SICI:
1398-9219(200011)1:11<852:TPOCFT>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANS-GOLGI NETWORK; ENDOPLASMIC-RETICULUM; INTERMEDIATE COMPARTMENT; CONDUCTANCE REGULATOR; SECRETORY PATHWAY; PROTEIN-TRANSPORT; DELTA-F508 CFTR; VESICULAR TRANSPORT; SELECTIVE EXPORT; MEMBRANE-PROTEIN;
Keywords:
CFTR; cystic fibrosis; cystic fibrosis transmembrane regulator; endoplasmic reticulum; Golgi vesicle;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
95
Recensione:
Indirizzi per estratti:
Indirizzo: Balch, WE Scripps Clin & Res Inst, Dept Cell, 10550 N Torrey Pines Rd, La Jolla, CA 92037 USA Scripps Clin & Res Inst 10550 N Torrey Pines Rd La Jolla CA USA 92037
Citazione:
S.I. Bannykh et al., "Traffic pattern of cystic fibrosis transmembrane regulator through the early exocytic pathway", TRAFFIC, 1(11), 2000, pp. 852-870

Abstract

The pathway of transport of the cystic fibrosis transmembrane regulator (CFTR) through the early exocytic pathway has not been examined. In contrast to most membrane proteins that are concentrated during export from the ER and therefore readily detectable at elevated revels in pre-Golgi intermediates and Golgi compartments, wild-type CFTR could not be detected in these compartments using deconvolution immunofluorescence microscopy. To determine the basis for this unusual feature, we analyzed CFTR localization using quantitative immunoelectron microscopy (IEM). We found that wild-type CFTR is present in pre-Golgi compartments and peripheral tubular elements associated with the cis and trans faces of the Golgi stack, albeit at a concentration 2-fold lower than that found in the endoplasmic reticulum (ER). Delta F508 CFTR, a mutant form that is not efficiently delivered to the cell surfaceand the most common mutation in cystic fibrosis, could also be detected ata reduced concentration in pre-Golgi intermediates and peripheral cis Golgi elements, but not in post-Golgi compartments. Our results suggest that the low level of wild-type CFTR in the Golgi region reflects a limiting step in selective recruitment by the ER export machinery, an event that is largely deficient in Delta F508. We raise the possibility that novel modes of selective anterograde and retrograde traffic between the ER and the Golgi mayserve to regulate CFTR function in the early secretory compartments.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 02:29:27