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Titolo:
A mushroom (Agaricus bisporus) tissue homogenate based alcohol oxidase electrode for alcohol determination in serum
Autore:
Akyilmaz, E; Dinckaya, E;
Indirizzi:
Ege Univ, Fac Sci, Dept Biochem, TR-35100 Bornova Izmir, Turkey Ege Univ Bornova Izmir Turkey TR-35100 m, TR-35100 Bornova Izmir, Turkey
Titolo Testata:
TALANTA
fascicolo: 3, volume: 53, anno: 2000,
pagine: 505 - 509
SICI:
0039-9140(200012)53:3<505:AM(BTH>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
CARBON-PASTE; ETHANOL; BIOSENSORS; PLANT; METHANOL; GLUCOSE; REACTOR;
Keywords:
ethyl alcohol; alcohol oxidase; biosensor; mushroom (Agaricus bisporus); tissue homogenate based electrode;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Dinckaya, E Ege Univ, Fac Sci, Dept Biochem, TR-35100 Bornova Izmir, Turkey Ege Univ Bornova Izmir Turkey TR-35100 Bornova Izmir, Turkey
Citazione:
E. Akyilmaz e E. Dinckaya, "A mushroom (Agaricus bisporus) tissue homogenate based alcohol oxidase electrode for alcohol determination in serum", TALANTA, 53(3), 2000, pp. 505-509

Abstract

To construct homogenized tissue based biosensors by using plant tissue materials is a relatively new development in the biosensor technology. In thisstudy, a homogenized mushroom (Agaricus bisporus) tissue based electrode in which alcohol oxidase activity was developed by immobilizing with gelatinand cross-linking agent glutaraldehyde at dissolved oxygen probe for determination of ethyl alcohol. The electrode response depends linearly on ethylalcohol concentration between 0.2 and 20 mM. In the optimization studies, phosphate buffer (pH 7.5. 50 mM) and 35 degreesC were obtained as the optimum conditions. By using the electrode prepared we have made approximately 60 measurements in 10-h period, and response time of the electrode is 2 min for each measurement. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 00:26:14