Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
UVA1 irradiation induces deoxyribonuclease dependent apoptosis in cutaneous T-cell lymphoma in vivo
Autore:
von Kobyletzki, G; Heine, O; Stephan, H; Pieck, C; Stucker, M; Hoffmann, K; Altmeyer, P; Mannherz, HG;
Indirizzi:
Ruhr Univ Bochum, St Josef Hosp, Dept Dermatol, D-44791 Bochum, Germany Ruhr Univ Bochum Bochum Germany D-44791 ermatol, D-44791 Bochum, Germany
Titolo Testata:
PHOTODERMATOLOGY PHOTOIMMUNOLOGY & PHOTOMEDICINE
fascicolo: 6, volume: 16, anno: 2000,
pagine: 271 - 277
SICI:
0905-4383(200012)16:6<271:UIIDDA>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
INDUCED LIPID-PEROXIDATION; HUMAN SKIN FIBROBLASTS; MYCOSIS-FUNGOIDES; ATOPIC-DERMATITIS; DNASE-GAMMA; EXPRESSION; RADIATION; DEATH; PHOTOTHERAPY; THERAPY;
Keywords:
apoptosis; cutaneous T-cell lymphoma (CTCL); deoxyribonuclease I (DNase I); in situ nick end-labeling of fragmented DNA (ISEL); Hoechst 33342;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: von Kobyletzki, G Ruhr Univ Bochum, St Josef Hosp, Dept Dermatol, Gudrunstr 56, D-44791 Bochum, Germany Ruhr Univ Bochum Gudrunstr 56 Bochum GermanyD-44791 ny
Citazione:
G. von Kobyletzki et al., "UVA1 irradiation induces deoxyribonuclease dependent apoptosis in cutaneous T-cell lymphoma in vivo", PHOTODERM P, 16(6), 2000, pp. 271-277

Abstract

Cutaneous T-cell lymphoma (CTCL) is a malignancy of mature T-cells, predominantly of the helper phenotype, that primarily invade the skin. Different photo-and chemotherapeutic treatments are known to be beneficial in early-stage CTCL, This observation has initiated prospective investigations into the efficacy of phototherapeutic regimens. The purpose of our study was to investigate the ability of medium-dose UVA1 phototherapy (60 J/cm(2)) to induce apoptosis (programmed cell death) in skin infiltrating T-cells of CTCL in vivo. We describe the results of three different staining methods for formalin-fixed, paraffin-embedded tissue sections. The in situ end-labeling (ISEL) procedure, nuclear staining using the DNA-binding fluorochrome Hoechst 33342, and immunohistochemistry using polyclonal antibodies against recombinant mouse deoxyribonuclease I (DNase I) demonstrated that UVA1 irradiation was able to induce marked apoptosis in CTCL. Thereby, ISEL and Hoechst staining clearly revealed DNA-condensation and nuclear fragmentation, accompanied by the formation of typical "apoptotic bodies". The accumulation of DNase I immunoreactivity in the cytoplasm of lymphocytes in UVA1 irradiated skin indicated that DNase I or DNase I-related endonucleases may have actedas apoptotic endonuclease(s) which were synthesized after UVA1 irradiationprior to their apoptotic elimination.

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Documento generato il 19/07/18 alle ore 13:24:25