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Titolo:
Parallel secretion of pancreatic phospholipase A(2), phospholipase A(1), lipase, and colipase in children with exocrine pancreatic dysfunction
Autore:
Nouri-Sorkhabi, MH; Chapman, BE; Kuchel, PW; Gruca, MA; Gaskin, KJ;
Indirizzi:
New Childrens Hosp, James Fairfax Inst Paediat Nutr, Westmead, NSW 2145, Australia New Childrens Hosp Westmead NSW Australia 2145 tmead, NSW 2145, Australia Univ Sydney, Dept Biochem, Sydney, NSW 2006, Australia Univ Sydney SydneyNSW Australia 2006 iochem, Sydney, NSW 2006, Australia
Titolo Testata:
PEDIATRIC RESEARCH
fascicolo: 6, volume: 48, anno: 2000,
pagine: 735 - 740
SICI:
0031-3998(200012)48:6<735:PSOPPA>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
SALT-STIMULATED LIPASE; ERYTHROCYTE-MEMBRANES; CYSTIC-FIBROSIS; P-31 NMR; BILE; INSUFFICIENCY; STEATORRHEA; ENZYMES; PLASMA; FAT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Gaskin, KJ New Childrens Hosp, James Fairfax Inst Paediat Nutr, Westmead, NSW 2145, Australia New Childrens Hosp Westmead NSW Australia 2145 2145, Australia
Citazione:
M.H. Nouri-Sorkhabi et al., "Parallel secretion of pancreatic phospholipase A(2), phospholipase A(1), lipase, and colipase in children with exocrine pancreatic dysfunction", PEDIAT RES, 48(6), 2000, pp. 735-740

Abstract

The cosecretion of pancreatic lipase and colipase are important in normal fat digestion. As adsorption of phosphatidylcholine to the lipid substrate interferes with lipase activity, hydrolysis to lysophosphatidylcholine withsubsequent desorption is also essential for fat digestion. Then are some data regarding the secretion of pancreatic phospholipases in normal adults but none in children or patients with pancreatic disease. In the present study, we aimed a) to develop an accurate fast assay method to measure phospholipase A(2) and b) to determine the secretion rate of pancreatic phospholipase A(2) and whether it is cosecreted with lipase and colipase in children with exocrine pancreatic dysfunction. Nine male patients aged 0.5 to 16 y (seven with cystic fibrosis, two with malabsorption) underwent pancreatic stimulation tests. Their colipase and lipase secretion rates were measured bytitrimetric methods and phospholipase A(2) and A(1) by phosphorus magneticresonance spectroscopy (P-31 NMR). It was found that the phospholipases, colipase, and lipase were absent in the two patients with pancreatic insufficiency. In patients with normal absorption, there were marked inter-and intrasubject variations of lipase, colipase, and phospholipase secretion ratesthat were consistent with the degree of exocrine pancreatic dysfunction. However, in the three 20-min stimulation periods of the pancreatic function test, pancreatic phospholipase is cosecreted with lipase and colipase, and average colipase and phospholipase A(2) secretion rates follow a similar orparallel pattern. These findings are consistent with the important role ofpancreatic phospholipases in intestinal phospholipid hydrolysis leading tothe desorption of phospholipids from the lipid substrate and enhancing lipid hydrolysis and phospholipid absorption.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 07:09:01