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Titolo:
Adult spinal cord stem cells generate neurons after transplantation in theadult dentate gyrus
Autore:
Shihabuddin, LS; Horner, PJ; Ray, J; Gage, FH;
Indirizzi:
Salk Inst Biol Studies, Genet Lab, La Jolla, CA 92037 USA Salk Inst Biol Studies La Jolla CA USA 92037 Lab, La Jolla, CA 92037 USA
Titolo Testata:
JOURNAL OF NEUROSCIENCE
fascicolo: 23, volume: 20, anno: 2000,
pagine: 8727 - 8735
SICI:
0270-6474(200012)20:23<8727:ASCSCG>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIBROBLAST GROWTH-FACTOR; CENTRAL-NERVOUS-SYSTEM; ELECTRON-MICROSCOPIC ANALYSIS; NEURAL PROGENITOR CELLS; PRECURSOR CELLS; IN-VIVO; LIGHT AUTORADIOGRAPHS; SUBVENTRICULAR ZONE; MAMMALIAN FOREBRAIN; MOUSE BRAIN;
Keywords:
spinal cord; stem cells; FGF; transplantation; neuroplasticity; adult;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Gage, FH Salk Inst Biol Studies, Genet Lab, 10010 N Torrey Pines Rd, La Jolla, CA 92037 USA Salk Inst Biol Studies 10010 N Torrey Pines Rd La Jolla CA USA 92037
Citazione:
L.S. Shihabuddin et al., "Adult spinal cord stem cells generate neurons after transplantation in theadult dentate gyrus", J NEUROSC, 20(23), 2000, pp. 8727-8735

Abstract

The adult rat spinal cord contains cells that can proliferate and differentiate into astrocytes and oligodendroglia in situ. Using clonal and subclonal analyses we demonstrate that, in contrast to progenitors isolated from the adult mouse spinal cord with a combination of growth factors, progenitors isolated from the adult rat spinal cord using basic fibroblast growth factor alone display stem cell properties as defined by their multipotentiality and self-renewal. Clonal cultures derived from single founder cells generate neurons, astrocytes, and oligodendrocytes, confirming the multipotent nature of the parent cell. Subcloning analysis showed that after serial passaging, recloning, and expansion, these cells retained multipotentiality, indicating that they are self-renewing. Transplantation of an in vitro-expanded clonal population of cells into the adult rat spinal cord resulted in their differentiation into glial cells only. However, after heterotopic transplantation into the hippocampus, transplanted cells that integrated in the granular cell layer differentiated into cells characteristic of this region, whereas engraftment into other hippocampal regions resulted in the differentiation of cells with astroglial and oligodendroglial phenotypes. The data indicate that clonally expanded, multipotent adult progenitor cells from a non-neurogenic region are not lineage-restricted to their developmental origin but can generate region-specific neurons in vivo when exposed to the appropriate environmental cues.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 08:32:06