Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
p0071, a member of the armadillo multigene family, is a constituent of sarcomeric I-bands in human skeletal muscle
Autore:
Schroder, R; Van der Ven, PFM; Warlo, I; Schumann, H; Furst, DO; Blumcke, I; Schmidt, MC; Hatzfeld, M;
Indirizzi:
Univ Halle Wittenberg, Fac Med, Mol Biol Grp, Halle, Germany Univ Halle Wittenberg Halle Germany c Med, Mol Biol Grp, Halle, Germany Univ Hosp Bonn, Dept Neurol, Bonn, Germany Univ Hosp Bonn Bonn GermanyUniv Hosp Bonn, Dept Neurol, Bonn, Germany Univ Potsdam, Dept Cell Biol, Potsdam, Germany Univ Potsdam Potsdam Germany Potsdam, Dept Cell Biol, Potsdam, Germany Univ Hosp Mainz, Dept Neuropathol, Mainz, Germany Univ Hosp Mainz Mainz Germany p Mainz, Dept Neuropathol, Mainz, Germany Univ Hosp Bonn, Dept Neuropathol, Bonn, Germany Univ Hosp Bonn Bonn Germany Hosp Bonn, Dept Neuropathol, Bonn, Germany Univ Hosp Bonn, Dept Neurosurg, Bonn, Germany Univ Hosp Bonn Bonn Germany iv Hosp Bonn, Dept Neurosurg, Bonn, Germany
Titolo Testata:
JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY
fascicolo: 6, volume: 21, anno: 2000,
pagine: 577 - 586
SICI:
0142-4319(200008)21:6<577:PAMOTA>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECESSIVE NEMALINE MYOPATHY; TRANSCRIPTION FACTOR LEF-1; BETA-CATENIN; DESMOSOMAL PLAQUE; NUCLEAR-LOCALIZATION; E-CADHERIN; MONOCLONAL-ANTIBODIES; PROTEIN INTERACTIONS; EPITHELIAL-CELLS; EMBRYONIC HEART;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
60
Recensione:
Indirizzi per estratti:
Indirizzo: Hatzfeld, M Univ Halle Wittenberg, Fac Med, Mol Biol Grp, Halle, Germany Univ Halle Wittenberg Halle Germany iol Grp, Halle, Germany
Citazione:
R. Schroder et al., "p0071, a member of the armadillo multigene family, is a constituent of sarcomeric I-bands in human skeletal muscle", J MUSCLE R, 21(6), 2000, pp. 577-586

Abstract

p0071 is a member of the armadillo gene family that is expressed in a widevariety of mammalian tissues and cell types with a prominent cell-cell contact association in epithelial cells. Here, we report the expression and localization patterns of p0071 in differentiating human skeletal muscle cellsand in normal and diseased human skeletal muscle tissues. Northern blots revealed expression of p0071 mRNA in adult skeletal muscle tissue. RT-PCR analysis and Western blotting experiments identified two differentially spliced isoforms of p0071. The balance between these isoforms shifted during in vitro differentiation of isolated muscle cells from predominant expression of the short variant to a preponderance of the larger variant from day 6 onwards. Immunolocalization studies in mature skeletal muscle tissue revealedthat p0071 is a constituent of myofibrils with a distinct localization at the level of sarcomeric N2-lines. During myofibrillogenesis, p0071 was not detected in non-striated nascent myofibrils, but became apparent shortly after the development of compact Z-discs in early myotubes. Furthermore, we studied the expression of p0071 in a wide variety of neuromuscular disordersby indirect immunofluorescence. Here, the myofibrillar staining of p0071 was preserved in all the disease entities included in our study. Our resultsprovide the first evidence that a member of the armadillo multigene familyis a constituent of the contractile apparatus in human skeletal muscle. The localization of p0071 at the level of I-bands and the timepoint of its integration into developing myofibrils suggest a possible role in the organization of thin filaments.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 12:57:31