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Titolo:
Confocal microscopic study of glial-vascular relationships in the retinas of pigmented rats
Autore:
Zahs, KR; Wu, T;
Indirizzi:
Univ Minnesota, Sch Med, Dept Physiol, Minneapolis, MN 55455 USA Univ Minnesota Minneapolis MN USA 55455 hysiol, Minneapolis, MN 55455 USA
Titolo Testata:
JOURNAL OF COMPARATIVE NEUROLOGY
fascicolo: 2, volume: 429, anno: 2001,
pagine: 253 - 269
SICI:
0021-9967(20010108)429:2<253:CMSOGR>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
BLOOD-BRAIN-BARRIER; GAP JUNCTION PERMEABILITY; WISTAR-KYOTO RATS; OPTIC-NERVE HEAD; MAMMALIAN RETINA; ENDOTHELIAL-CELLS; ASTROCYTE ENDFEET; HORIZONTAL CELLS; DOPAMINERGIC MODULATION; INOSITOL-TRISPHOSPHATE;
Keywords:
astrocyte; astroglia; Muller cell; gap junction; Lucifer yellow; neurobiotin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
79
Recensione:
Indirizzi per estratti:
Indirizzo: Zahs, KR Univ Minnesota, Sch Med, Dept Physiol, 6-125 Jackson,321 Church St SE, Minneapolis, MN 55455 USA Univ Minnesota 6-125 Jackson,321 Church St SE Minneapolis MN USA 55455
Citazione:
K.R. Zahs e T. Wu, "Confocal microscopic study of glial-vascular relationships in the retinas of pigmented rats", J COMP NEUR, 429(2), 2001, pp. 253-269

Abstract

Astroglia are interposed between the cerebral vasculature and neurons, where they may mediate the transfer of substances from the circulation to neurons and couple changes in neuronal activity to changes in cerebral blood flow. The retina is a particularly advantageous model system for studying glial-vascular interactions in situ. Confocal microscopy and three-dimensionalimage reconstruction were used to study the anatomical relationships between glia and the surface vasculature in retinas acutely isolated from adult pigmented rats. Retinas were immunostained using antibodies directed against the basal lamina surrounding the vasculature as well as antibodies directed against glial fibrillary acidic protein. Surface vessels of all caliberswere contacted by the processes of astrocytes. The vitreal surfaces of thelarge retinal vessels were covered by a meshwork of immunoreactive astrocyte processes of a variety of shapes, whereas the scleral surfaces of the vessels were supported by thick bundles of astrocyte processes. In addition, glial cells were filled intracellularly with the gap junction-permeable tracers Lucifer yellow and Neurobiotin. Intracellular fills dearly demonstrated the presence of astrocytes with somata that were closely apposed to the large retinal vessels. Tracer-filled astrocytes displayed a variety and complexity of shapes that was not apparent in immunostained material. Gap junctional coupling was stronger between astrocytes adjacent to the same artery than between periarterial astrocytes and astrocytes located away from arteries. Significantly fewer Muller cells were labeled when Neurobiotin was injected into astrocytes associated with arteries than when Neurobiotin was injected into astrocytes that were distant from arteries. J. Comp. Neurol. 429:253-269, 2001. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/01/20 alle ore 07:01:10