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Titolo:
Immunoassay-based determination of phenobarbital using size-exclusion chromatography
Autore:
Bereczki, A; Horvath, V; Horvai, G;
Indirizzi:
Budapest Univ Technol & Econ, Inst Gen & Analyt Chem, H-1111 Budapest, Hungary Budapest Univ Technol & Econ Budapest Hungary H-1111 1 Budapest, Hungary Budapest Univ Technol & Econ, Div Chem Informat Technol, H-1111 Budapest, Hungary Budapest Univ Technol & Econ Budapest Hungary H-1111 1 Budapest, Hungary
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 2, volume: 749, anno: 2000,
pagine: 215 - 223
SICI:
1387-2273(200012)749:2<215:IDOPUS>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; DIRECT SERUM INJECTION; FLUORESCENCE POLARIZATION IMMUNOASSAY; ANTIEPILEPTIC DRUGS; ANTICONVULSANT DRUGS; PHENYTOIN; CARBAMAZEPINE; METABOLITES; IMMUNOANALYSIS; PLASMA;
Keywords:
phenobarbital; fluorescent immunoassay; size-exclusion chromatography;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Horvath, V Budapest Univ Technol & Econ, Inst Gen & Analyt Chem, Gellert Ter 4, H-1111 Budapest, Hungary Budapest Univ Technol & Econ Gellert Ter 4 Budapest Hungary H-1111
Citazione:
A. Bereczki et al., "Immunoassay-based determination of phenobarbital using size-exclusion chromatography", J CHROMAT B, 749(2), 2000, pp. 215-223

Abstract

The measurement of the anti-epileptic drug phenobarbital from serum samples combining immunoassay and size-exclusion chromatography is presented. Theimmunoreaction is based on the competitive binding of the analyte (unlabelled phenobarbital) and the fluorescent-labelled phenobarbital to anti-phenobarbital antibodies. Mixing of the reagents and the immunoreaction takes place in a Row system. The products are separated on-line on a short gel chromatographic column and the fluorescence intensity of the marker is measured. The calibration curve shows good linearity in the range 5-80 mug/ml, corresponding to therapeutically relevant serum levels. Intra-day precision values are between 7.32 and 9.48%; the accuracy is between 0.97 and 9.43%. Inter-day precision and accuracy measured on 6 different days fall between 5.38 and 10.05% and -8.27 and -4.97%, respectively. The results obtained with the proposed method show a good correlation with those of other methods (radioimmunoassay and fluorescence polarisation immunoassay) already established in clinical laboratories. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 19:58:52