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Titolo:
The fission yeast Ran GTPase is required for microtubule integrity
Autore:
Fleig, U; Salus, SS; Karig, I; Sazer, S;
Indirizzi:
Univ Dusseldorf, Inst Mikrobiol, D-40225 Dusseldorf, Germany Univ Dusseldorf Dusseldorf Germany D-40225 , D-40225 Dusseldorf, Germany Baylor Coll Med, Dept Biochem & Mol Biol, Houston, TX 77030 USA Baylor Coll Med Houston TX USA 77030 em & Mol Biol, Houston, TX 77030 USA
Titolo Testata:
JOURNAL OF CELL BIOLOGY
fascicolo: 5, volume: 151, anno: 2000,
pagine: 1101 - 1111
SICI:
0021-9525(20001127)151:5<1101:TFYRGI>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
BIPOLAR SPINDLE FORMATION; TRANSCRIPTION FACTOR PAP1; XENOPUS EGG EXTRACTS; SCHIZOSACCHAROMYCES-POMBE; CELL-CYCLE; CHROMOSOME SEGREGATION; OXIDATIVE STRESS; BOUND RAN; POLE BODY; PROTEIN;
Keywords:
Ran GTPase; microtubules; chromosome segregation; mitosis; fission yeast;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Fleig, U Univ Dusseldorf, Inst Mikrobiol, Univ Str 1, D-40225 Dusseldorf, Germany Univ Dusseldorf Univ Str 1 Dusseldorf Germany D-40225 f, Germany
Citazione:
U. Fleig et al., "The fission yeast Ran GTPase is required for microtubule integrity", J CELL BIOL, 151(5), 2000, pp. 1101-1111

Abstract

The microtubule cytoskeleton plays a pivotal role in cytoplasmic organization, cell division, and the correct transmission of genetic information. Ina screen designed to identify fission yeast genes required for chromosome segregation, we identified a strain that carries a point mutation in the SpRan GTPase. Ran is an evolutionarily conserved eukaryotic GTPase that directly participates in nucleocytoplasmic transport and whose loss affects manybiological processes. Recently a transport-independent effect of Ran on spindle formation in vitro was demonstrated, but the in vivo relevance of these findings was unclear. Here, we report the characterization of a Schizosaccharomyces pombe Ran GTPase partial loss of function mutant in which nucleocytoplasmic protein transport is normal, but the microtubule cytoskeleton is defective, resulting in chromosome mis-segregation and abnormal cell shape. These abnormalities are exacerbated by microtubule destabilizing drugs,by loss of the spindle checkpoint protein Mph1p, and by mutations in the spindle pole body component Cut11p, indicating that SpRan influences microtubule integrity. As the SpRan mutant phenotype can be partially suppressed by the presence of extra Mal3p, we suggest that SpRan plays a role in microtubule stability.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 00:49:05