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Titolo:
LiSa-2, a novel human liposarcoma cell line with a high capacity for terminal adipose differentiation
Autore:
Wabitsch, M; Bruderlein, S; Melzner, I; Braun, M; Mechtersheimer, G; Moller, P;
Indirizzi:
Univ Ulm, Dept Pathol, D-89081 Ulm, Germany Univ Ulm Ulm Germany D-89081Univ Ulm, Dept Pathol, D-89081 Ulm, Germany Univ Ulm, Dept Pediat, D-89081 Ulm, Germany Univ Ulm Ulm Germany D-89081Univ Ulm, Dept Pediat, D-89081 Ulm, Germany
Titolo Testata:
INTERNATIONAL JOURNAL OF CANCER
fascicolo: 6, volume: 88, anno: 2000,
pagine: 889 - 894
SICI:
0020-7136(200012)88:6<889:LANHLC>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
COMPARATIVE GENOMIC HYBRIDIZATION; ADIPOCYTE PRECURSOR CELLS; ACTIVATED RECEPTOR-GAMMA; DNA DEMETHYLATION; LOSSES; GAINS; GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Moller, P Univ Ulm, Dept Pathol, Albert Einstein Allee 11, D-89081 Ulm, Germany Univ Ulm Albert Einstein Allee 11 Ulm Germany D-89081 , Germany
Citazione:
M. Wabitsch et al., "LiSa-2, a novel human liposarcoma cell line with a high capacity for terminal adipose differentiation", INT J CANC, 88(6), 2000, pp. 889-894

Abstract

LiSa-2 is a stable cell line derived from a poorly differentiated, pleomorphic liposarcoma, In serum-containing medium, LiSa-2 cells are fibroblastoid and rapidly dividing. In a serum-tree, chemically defined culture medium containing physiological concentrations of insulin, triiodothyronine and cortisol, LiSa-2 cells divide slower and, extensively storing fat, acquire adipocyte morphology, In contrast to fibroblastoid LiSa-2 cells, these adipocyte-like LiSa-2 cells highly express transcripts for peroxisome proliferator-activated receptor-gamma, lipoprotein lipase, fatty acid synthetase, hormone-sensitive lipase, adipocyte most abundant gene transcript-1, glycerol-3-phosphate-dehydrogenase and the insulin-sensitive glucose transporter-4, all of which are specific for differentiated adipocytes. However, leptin mRNA expression was demonstrated only after preventing DNA methylation by incorporation of 5-aza-deoxycytidine into cellular DNA. Functionally, adipocyte-like LiSa-2 cells show increased insulin-dependent glucose uptake and lipid synthesis and are sensitive to lipolytic agents. This cell line may serveas an in vitro model for studying the regulation of human liposarcoma differentiation and for screening drugs for induction of differentiation-associated growth arrest in liposarcomas. Int. J. Cancer 88:889-894, 2000. (C) 2000 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 14:43:37