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Titolo:
Monoclonal antibody DS6 detects a tumor-associated sialoglycotope expressed on human serous ovarian carcinomas
Autore:
Kearse, KP; Smith, NL; Semer, DA; Eagles, L; Finley, JL; Kazmierczak, S; Kovacs, CJ; Rodriguez, AA; Kellogg-Wennerberg, AE;
Indirizzi:
E Carolina Univ, Brody Sch Med, Dept Pathol & Lab Med, Greenville, NC 27858 USA E Carolina Univ Greenville NC USA 27858 Lab Med, Greenville, NC 27858 USA E Carolina Univ, Brody Sch Med, Dept Microbiol & Immunol, Greenville, NC 27858 USA E Carolina Univ Greenville NC USA 27858 Immunol, Greenville, NC 27858 USA E Carolina Univ, Brody Sch Med, Dept Obstet & Gynecol, Div Gynecol Oncol, Greenville, NC 27858 USA E Carolina Univ Greenville NC USA 27858 l Oncol, Greenville, NC 27858 USA E Carolina Univ, Brody Sch Med, Dept Radiat Oncol, Greenville, NC 27858 USA E Carolina Univ Greenville NC USA 27858 t Oncol, Greenville, NC 27858 USA E Carolina Univ, Dept Chem, Greenville, NC 27858 USA E Carolina Univ Greenville NC USA 27858 pt Chem, Greenville, NC 27858 USA
Titolo Testata:
INTERNATIONAL JOURNAL OF CANCER
fascicolo: 6, volume: 88, anno: 2000,
pagine: 866 - 872
SICI:
0020-7136(200012)88:6<866:MADDAT>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
CANCER-ASSOCIATED ANTIGEN; HUMAN-TISSUES; BREAST; CELLS; IMMUNOHISTOCHEMISTRY; IDENTIFICATION; MARKERS; CA-125; SERA; LUNG;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Kellogg-Wennerberg, AE E Carolina Univ, Brody Sch Med, Dept Pathol & Lab Med, 7S10 Brody Bldg, Greenville, NC 27858 USA E Carolina Univ 7S10 Brody Bldg Greenville NC USA 27858
Citazione:
K.P. Kearse et al., "Monoclonal antibody DS6 detects a tumor-associated sialoglycotope expressed on human serous ovarian carcinomas", INT J CANC, 88(6), 2000, pp. 866-872

Abstract

A newly developed murine monoclonal antibody, DS6, immunohistochemically reacts with an antigen, CA6, that is expressed by human serous ovarian carcinomas but not by normal ovarian surface epithelium or mesothelium, CA6 has a limited distribution in normal adult tissues and is most characteristically detected in fallopian tube epithelium, inner urothelium and type 2 pneumocytes, Pre-treatment of tissue sections with either periodic acid or neuraminidase from Vibrio cholerae abolishes immunoreactivity with DS6, indicating that CA6 is a neuraminidase-sensitive and periodic acid-sensitive sialicacid glycoconjugate ("sialoglycotope"). SDS-PACE of OVCAR5 cell lysates has revealed that the CA6 epitope is expressed on an 80 kDa non-disulfide-linked glycoprotein containing N-linked oligosaccharides, Two-dimensional non-equilibrium pH gradient gel electrophoresis indicates an isoelectric point of approximately 6.2 to 6.5. Comparison of the immunohistochemical distribution of CA6 in human serous ovarian adenocarcinomas has revealed similarities to that of CA125; however, distinct differences and some complementarityof antigen expression were revealed by double-label, 2-color immunohistochemical studies. The DS6-detected CA6 antigen appears to be distinct from other well-characterized tumor-associated antigens, including MUC1, CA125 andthe histo-blood group-related antigens sLea, sLex and sTn, Int, J, Cancer 88:866-872, 2000, (C) 2000 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 12:47:58