Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Possible involvement of the adenine nucleotide translocase in the activation of the permeability transition pore induced by cadmium
Autore:
Zazueta, C; Sanchez, C; Garcia, N; Correa, F;
Indirizzi:
Inst Nacl Cardiol, Dept Bioquim, Mexico City 014080, DF, Mexico Inst Nacl Cardiol Mexico City DF Mexico 014080 co City 014080, DF, Mexico
Titolo Testata:
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
fascicolo: 10, volume: 32, anno: 2000,
pagine: 1093 - 1101
SICI:
1357-2725(200010)32:10<1093:PIOTAN>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT-LIVER MITOCHONDRIA; ADP/ATP CARRIER; CROSS-LINKING; MEMBRANE-PERMEABILITY; PHENYLARSINE OXIDE; TRANSPORT ACTIVITY; PLUS PROOXIDANTS; PROTEIN; BINDING; CYCLOSPORINE;
Keywords:
permeability transition; ADP/ATP carrier; calcium efflux; cadmium; mitochondria;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Zazueta, C Inst Nacl Cardiol, Dept Bioquim, Ignacio Chavez,Juan Badiano 1,Colonia Secc 16, Mexico City 014080, DF, Mexico Inst Nacl Cardiol Ignacio Chavez,Juan Badiano 1,Colonia Secc 16 Mexico City DF Mexico 014080
Citazione:
C. Zazueta et al., "Possible involvement of the adenine nucleotide translocase in the activation of the permeability transition pore induced by cadmium", INT J BIO C, 32(10), 2000, pp. 1093-1101

Abstract

Low levels of cadmium induce a rapid calcium efflux in energized rat kidney mitochondria. This is accompanied by the collapse of the transmembrane gradient in a partial CSA-sensitive fashion. The binding of Cd-109(2+) to mitochondria is a saturable function; in the presence of NEM, the binding of 2.5 nmol Cd-109(2+)/mg of protein suffices to induce the opening of the permeability transition pore. It was found that cadmium bound mainly to proteins of molecular weight between 30 and 50 kDa. In the presence of the monothiol reagent NEM, the label is concentrated in the 30 kDa protein. Following the addition of the reducing agent dithiothreitol, calcium is reaccumulatedand the membrane potential restored. This correlates with a significant loss of label in the 30 kDa protein region. The 30 kDa protein was identifiedas the adenine nucleotide translocase by labelling experiments with eosin 5-maleimide and experiments of reconstitution. (C) 2000 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 13:02:14