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Titolo:
Histochemical visualization of oxidant stress
Autore:
Frank, J; Pompella, A; Biesalski, HK;
Indirizzi:
Univ Hohenheim, Dept Biol Chem & Nutr, D-70593 Stuttgart, Germany Univ Hohenheim Stuttgart Germany D-70593 utr, D-70593 Stuttgart, Germany Univ Siena, Dept Pathophysiol & Expt Med, I-53100 Siena, Italy Univ SienaSiena Italy I-53100 ophysiol & Expt Med, I-53100 Siena, Italy
Titolo Testata:
FREE RADICAL BIOLOGY AND MEDICINE
fascicolo: 11, volume: 29, anno: 2000,
pagine: 1096 - 1105
SICI:
0891-5849(200012)29:11<1096:HVOOS>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOW-DENSITY-LIPOPROTEIN; GLYCATION END-PRODUCTS; IN-SITU DETECTION; LIPID-PEROXIDATION; MODIFIED PROTEINS; OXIDATIVE DAMAGE; IMMUNOHISTOCHEMICAL DETECTION; HEPATOCELLULAR INJURY; CONFOCAL MICROSCOPY; ALZHEIMERS-DISEASE;
Keywords:
free radicals; histochemistry; lipid peroxidation; protein oxidation; nitrosylated proteins;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
73
Recensione:
Indirizzi per estratti:
Indirizzo: Biesalski, HK Univ Hohenheim, Dept Biol Chem & Nutr, Fruwirthstrassen 12, D-70593 Stuttgart, Germany Univ Hohenheim Fruwirthstrassen 12 Stuttgart Germany D-70593
Citazione:
J. Frank et al., "Histochemical visualization of oxidant stress", FREE RAD B, 29(11), 2000, pp. 1096-1105

Abstract

Free radicals induce oxidative modification in distinct components of the living matter (lipid, proteins, and DNA). For qualitative and quantitative determination of free radical-induced modifications, different, more or less sensitive biochemical methods are available. Because of the high reactivity and short life of free radicals, ongoing oxidative damage is generally analyzed by measurement of secondary products-such as H2O2, oxidized proteins, peroxidized lipids, and their breakdown products, oxidized DNA-or by fluorographic analysis in combination with fluorescent dyes such as dichlorofluorescin (DCFH). In addition, the determination of free radical-related oxidation products is usually carried out in plasma, urine, or, less frequently, in bioptic material. Consequently, biochemical data seldom reflect the effects of free radical insults in situ. The histochemical visualization of selected molecular markers of oxidative damage can often provide more valuable information concerning the in vivo distribution of oxidative processes. This review summarizes the methods currently available for histochemical detection and indirect visualization of free radical-induced alterations in tissues and isolated cells. (C) 2000 Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 12:37:08