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Titolo:
Numerical density of mu opioidreceptor expressing neurons in the frontal cortex of drug related fatalities
Autore:
Schmidt, P; Schmolke, C; Musshoff, F; Prohaska, C; Menzen, M; Madea, B;
Indirizzi:
Univ Bonn, Inst Forens Med, D-53111 Bonn, Germany Univ Bonn Bonn GermanyD-53111 n, Inst Forens Med, D-53111 Bonn, Germany Rhein Kliniken Bonn, D-53111 Bonn, Germany Rhein Kliniken Bonn Bonn Germany D-53111 ken Bonn, D-53111 Bonn, Germany
Titolo Testata:
FORENSIC SCIENCE INTERNATIONAL
fascicolo: 3, volume: 115, anno: 2001,
pagine: 219 - 229
SICI:
0379-0738(20010115)115:3<219:NDOMOE>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECEPTOR MESSENGER-RNA; OPIOID-RECEPTOR; DOWN-REGULATION; RAT-BRAIN; OPIATE RECEPTORS; CEREBRAL-CORTEX; MORPHINE; BINDING; CELLS; CNS;
Keywords:
human cerebral cortex; drug-related fatalities of morphine-addicts; mu opioidreceptor; immunohistochemistry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Madea, B Univ Bonn, Inst Forens Med, Stiftspl 12, D-53111 Bonn, Germany Univ Bonn Stiftspl 12 Bonn Germany D-53111 D-53111 Bonn, Germany
Citazione:
P. Schmidt et al., "Numerical density of mu opioidreceptor expressing neurons in the frontal cortex of drug related fatalities", FOREN SCI I, 115(3), 2001, pp. 219-229

Abstract

In animal and cell culture experiments, chronic morphine treatment has been followed by 'up'- as well as 'down-regulation' of the mu opioidreceptor (mu OR) number. The present postmortem morphometric study of morphine-related fatalities of drug addicts (n = 12 and 22-35 years old, with blood unconjugated morphine levels from 27.1 to 458 ng/ml, m.v. 198.5 ng/ml) versus a non-addicted control group (n = 13 and 10-44 years old) was intended to examine whether chronic opiate exposure affects the numerical density of mu OR expressing neurons in the human neocortex (area 10 according to Brodmann). For the immunohistochemical procedure, thick (100 mum) vibratome sections were incubated with a monoclonal antibody against the mu OR [Arvidsson et al., J. Neurosci. 15 (1995) 3328] and immunoreactive sites were visualized using an immunoperoxidase protocol. The numerical densities of mu OR-expressing and Nissl-stained neurons were assessed morphometrically (camera lucida-drawings). In both collectives, the anti-mu OR immunoreactivity was mainly found in pyramidal neurons of layers (L) II/III and V and in multiform neurons of L VI. In the drug-related fatalities and the control group, the density of neurons expressing mu OR protein was similar, amounting for 2698 +/-153 and 2688 +/- 172/mm(3), respectively. These findings extend the binding studies of opioid ligands in postmortem brains of heroin addicts [Gabilondo ct al., Psychopharmacology 115 (1994) 135] revealing similar receptor densities and affinities by showing no difference in the density of mu OR-positive neurons. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 03:38:26