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Titolo:
BDNF, NT-3 and NGF induce distinct new Ca2+ channel synthesis in developing hippocampal neurons
Autore:
Baldelli, P; Forni, PE; Carbone, E;
Indirizzi:
Univ Turin, INFM Res Unit, I-10125 Turin, Italy Univ Turin Turin Italy I-10125 urin, INFM Res Unit, I-10125 Turin, Italy Univ Turin, Dept Neurosci, I-10125 Turin, Italy Univ Turin Turin Italy I-10125 urin, Dept Neurosci, I-10125 Turin, Italy Univ Turin, Dept Anat, Turin, Italy Univ Turin Turin ItalyUniv Turin, Dept Anat, Turin, Italy
Titolo Testata:
EUROPEAN JOURNAL OF NEUROSCIENCE
fascicolo: 11, volume: 12, anno: 2000,
pagine: 4017 - 4032
SICI:
0953-816X(200011)12:11<4017:BNANID>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
NERVE GROWTH-FACTOR; RAT EMBRYO MOTONEURONS; METHYL-D-ASPARTATE; NEUROTROPHIC FACTOR; CALCIUM CHANNELS; N-TYPE; SYNAPTIC TRANSMISSION; OMEGA-CONOTOXIN; NEUROTRANSMITTER RELEASE; INTRACELLULAR CALCIUM;
Keywords:
cultured rat embryo neurons; L- N- R- and P/Q-type channels; MAP-kinase; neurotrophins; Trk receptors;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
69
Recensione:
Indirizzi per estratti:
Indirizzo: Carbone, E Univ Turin, INFM Res Unit, Corso Raffaello 30, I-10125 Turin, Italy Univ Turin Corso Raffaello 30 Turin Italy I-10125 Turin, Italy
Citazione:
P. Baldelli et al., "BDNF, NT-3 and NGF induce distinct new Ca2+ channel synthesis in developing hippocampal neurons", EUR J NEURO, 12(11), 2000, pp. 4017-4032

Abstract

Neurotrophins exert short- and long-term effects on synaptic transmission. The mechanism underlying these forms of synaptic plasticity is unknown although it is likely that intracellular Ca2+ and presynaptic Ca2+ channels play a critical role. Here we show that BDNF, NGF and NT-3 (10-100 ng/mL) exhibit a selective long-term up-regulation of voltage-gated Ca2+ current densities in developing hippocampal neurons of 6-20 days in culture. NGF and NT-3 appear more effective in up-regulating L-currents, while BDNF predominantly acts on non-L-currents (N, P/Q and R). The effects of the three neurotrophins were time- and dose-dependent. The EC50 was comparable for BDNF, NGFand NT-3 (10-16 ng/mL) while the time of half-maximal activation was significantly longer for NGF compared to BDNF (58 vs. 25 h). Despite the increased Ca2+ current density, the neurotrophins did not alter the voltage-dependence of channel activation, the kinetics parameters or the elementary properties of Ca2+ channels (single-channel conductance, probability of opening and mean open time). Neurotrophin effects were completely abolished by coincubation with the nonspecific Trk-receptor inhibitor K252a, the protein synthesis blocker anisomycin and the MAP-kinase inhibitor PD98059, while cotreatment with the PLC-gamma blocker, U73122, was without effect. Immunocytochemistry and Western blotting revealed that neurotrophins induced an increased MAP-kinase phosphorylation and its translocation to the nucleus. The present findings suggest that on a long time scale different neurotrophins canselectively up-regulate different Ca2+ channels. The action is mediated byTrk-receptors/MAP-kinase pathways and induces an increased density of newly available Ca2+ channels with unaltered gating activity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 15:43:18