Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
The Nramp2/DMT1 iron transporter is induced in the duodenum of microcytic anemia mk mice but is not properly targeted to the intestinal brush border
Autore:
Canonne-Hergaux, F; Fleming, MD; Levy, JE; Gauthier, S; Ralph, T; Picard, V; Andrews, NC; Gros, P;
Indirizzi:
McGill Univ, Dept Biochem, Montreal, PQ H3G 1Y6, Canada McGill Univ Montreal PQ Canada H3G 1Y6 chem, Montreal, PQ H3G 1Y6, Canada Childrens Hosp, Div Hematol Oncol, Boston, MA 02115 USA Childrens Hosp Boston MA USA 02115 iv Hematol Oncol, Boston, MA 02115 USA Howard Hughes Med Inst, Boston, MA 02115 USA Howard Hughes Med Inst Boston MA USA 02115 Med Inst, Boston, MA 02115 USA Brigham & Womens Hosp, Div Hematol, Boston, MA 02115 USA Brigham & Womens Hosp Boston MA USA 02115 v Hematol, Boston, MA 02115 USA Brigham & Womens Hosp, Dept Pathol, Boston, MA 02115 USA Brigham & Womens Hosp Boston MA USA 02115 pt Pathol, Boston, MA 02115 USA Harvard Univ, Sch Med, Dept Pediat, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 h Med, Dept Pediat, Boston, MA 02115 USA
Titolo Testata:
BLOOD
fascicolo: 12, volume: 96, anno: 2000,
pagine: 3964 - 3970
SICI:
0006-4971(200012)96:12<3964:TNITII>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
NATURAL-RESISTANCE; INTRACELLULAR PARASITES; BILIARY GLYCOPROTEIN; NRAMP1 PROTEIN; GENE; INFECTION; ABSORPTION; EXPRESSION; MEMBRANE; METABOLISM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Gros, P McGill Univ, Dept Biochem, 3655 Drummond,Room 907, Montreal, PQ H3G 1Y6, Canada McGill Univ 3655 Drummond,Room 907 Montreal PQ Canada H3G 1Y6nada
Citazione:
F. Canonne-Hergaux et al., "The Nramp2/DMT1 iron transporter is induced in the duodenum of microcytic anemia mk mice but is not properly targeted to the intestinal brush border", BLOOD, 96(12), 2000, pp. 3964-3970

Abstract

Microcytic anemia (mk) mice and Belgrade (b) rats are severely iron deficient because of impaired intestinal iron absorption and defective iron metabolism in peripheral tissues. Both animals carry a glycine to arginine substitution at position 185 in the iron transporter known as Nramp2/DMT1 (divalent metal transporter 1). DMT1 messenger RNA (mRNA) and protein expression has been examined in the gastrointestinal tract of mk mice. Northern blot analysis indicates that, by comparison to mk/+ heterozygotes, mk/mk homozygotes show a dramatic increase in the level of DMT1 mRNA in the duodenum, This increase in RNA expression is paralleled by a concomitant increase of the100-kd DMT1 isoform I protein expression in the duodenum, Immunohistochemical analyses show that, as for normal mice on a low iron diet, DMT1 expression in enterocytes of mk/mk mice is restricted to the duodenum, However, and in contrast to normal enterocytes, little if any expression of DMT1 is seen at the apical membrane in mk/mk mice. These results suggest that the G185R mutation, which was shown to impair the transport properties of DMT1, also affects the membrane targeting of the protein in mk/mk enterocytes, Thisloss of function of DMT1 is paralleled by a dramatic increase in expression of the defective protein in mk/mk mice. This is consistent with a feedback regulation of DMT1 expression by iron stores. (C) 2000 by The American Society of Hematology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 10:09:39