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Titolo:
Hematopoietic stem cells express Tie-2 receptor in the murine fetal liver
Autore:
Hsu, HC; Ema, H; Osawa, M; Nakamura, Y; Suda, T; Nakauchi, H;
Indirizzi:
Univ Tsukuba, Inst Basic Med Sci, Dept Immunol, Tsukuba, Ibaraki 3058575, Japan Univ Tsukuba Tsukuba Ibaraki Japan 3058575 sukuba, Ibaraki 3058575, Japan Japan Sci & Technol Corp, CREST, Tsukuba, Ibaraki, Japan Japan Sci & Technol Corp Tsukuba Ibaraki Japan , Tsukuba, Ibaraki, Japan Kumamoto Univ, Sch Med, Dept Cell Differentiat, Inst Mol Embryol & Genet, Kumamoto 860, Japan Kumamoto Univ Kumamoto Japan 860 ol Embryol & Genet, Kumamoto 860, Japan
Titolo Testata:
BLOOD
fascicolo: 12, volume: 96, anno: 2000,
pagine: 3757 - 3762
SICI:
0006-4971(200012)96:12<3757:HSCETR>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADULT BONE-MARROW; TYROSINE KINASE; LONG-TERM; ENDOTHELIAL-CELLS; MOUSE EMBRYO; MOLECULAR CHARACTERIZATION; TEK RECEPTOR; YOLK-SAC; IN-VIVO; PRECURSORS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Nakauchi, H Univ Tsukuba, Inst Basic Med Sci, Dept Immunol, 1-1-1 Tennodai, Tsukuba, Ibaraki 3058575, Japan Univ Tsukuba 1-1-1 Tennodai Tsukuba Ibaraki Japan 3058575 apan
Citazione:
H.C. Hsu et al., "Hematopoietic stem cells express Tie-2 receptor in the murine fetal liver", BLOOD, 96(12), 2000, pp. 3757-3762

Abstract

Tie-2 receptor tyrosine kinase expressed in endothelial and hematopoietic cells is believed to play a role in both angiogenesis and hematopoietic during development of the mouse embryo. This article addressed whether Tie-2 is expressed on fetal liver hematopoietic stem cells (HSCs) at day 14 of gestation. With the use of anti-Tie-2 monoclonal antibody, its expression was detected in approximately 7% of an HSC population of Kit-positive, Sca-1-positive, lineage-negative or -low, and AA4.1-positive (KSLA) cells. These Tie-2-positive KSLA (T+ KSLA) cells rep resent 0.01% to 0.02% of fetal liver cells. In vitro colony and in vivo competitive repopulation assays were performed for T+ KSLA cells and Tie-2-negative KSLA (T- KSLA) cells, In the presence of stem cell factor, interleukin-3, and erythropoietin, 80% of T+ KSLA cells formed colonies in vitro, compared with 40% of T- KSLA cells. Long-term multilineage repopulating cells were detected in T+ KSLA cells, but not in T- KSLA cells. An in vive limiting dilution analysis revealed that atleast 1 of 8 T+ KSLA cells were such repopulating cells. The successful secondary transplantation initiated with a limited number of T+ KSLA cells suggests that these cells have self-renewal potential, In addition, engraftment of T+ KSLA cells in conditioned newborn mice indicates that these HSCs can be adapted equally by the adult and newborn hematopoietic environments. The data suggest that T+ KSLA cells represent HSCs in the murine fetal liver. (C) 2000 by The American Society of Hematology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 12:04:49