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Titolo:
Effects of pore size in 3-D fibrous matrix on human trophoblast tissue development
Autore:
Ma, T; Li, Y; Yang, ST; Kniss, DA;
Indirizzi:
Ohio State Univ, Dept Chem Engn, Columbus, OH 43210 USA Ohio State Univ Columbus OH USA 43210 t Chem Engn, Columbus, OH 43210 USA Ohio State Univ, Dept Obstet & Gynecol, Lab Perinatal Res, Columbus, OH 43210 USA Ohio State Univ Columbus OH USA 43210 rinatal Res, Columbus, OH 43210 USA
Titolo Testata:
BIOTECHNOLOGY AND BIOENGINEERING
fascicolo: 6, volume: 70, anno: 2000,
pagine: 606 - 618
SICI:
0006-3592(200012)70:6<606:EOPSI3>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN PLACENTAL-TRANSFER; ARTIFICIAL SKIN; POLYMER SCAFFOLDS; CELL-SHAPE; IN-VITRO; CULTURE; INHIBITOR; DESIGN; DIFFERENTIATION; EXPRESSION;
Keywords:
trophoblast; tissue engineering; 3-D culture; polyethylene terephthalate; fibrous matrix; proliferation; differentiation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Yang, ST Ohio State Univ, Dept Chem Engn, 140 W 19Th Ave, Columbus, OH 43210 USA Ohio State Univ 140 W 19Th Ave Columbus OH USA 43210 OH 43210 USA
Citazione:
T. Ma et al., "Effects of pore size in 3-D fibrous matrix on human trophoblast tissue development", BIOTECH BIO, 70(6), 2000, pp. 606-618

Abstract

The effects of pore size in a 3-D polyethylene terephthalate (PET) nonwoven fibrous matrix on longterm tissue development of human trophoblast ED27 cells were studied. Thermal compression was used to modify the porosity and pore size of the PET matrix. The pore size distributions in PET matrices were quantified using a liquid extrusion method. Cell metabolic activities, estradiol production, and cell proliferation and differentiation were studied for ED27 cells cultured in the thermally compressed PET matrices with known pore structure characteristics. In general, metabolic activities and proliferation rate were higher initially for cultures grown in the low-porosity (LP) PET matrix (porosity of 0.849, average pore size of 30 mum in diameter) than those in the high-porosity (HP) matrix (porosity of 0.896, averagepore size of 39 mum in diameter). However, 17 beta -estradiol production and cell differentiation activity in the HP matrix surpassed those in the LPmatrix after 12 days. The expression levels of cyclin B1 and p27(kip1) in cells revealed progressively decreasing proliferation and increasing differentiation activities for cells grown in PET matrices. Also, difference in pore size controlled the cell spatial organization in the PET matrices and contributed to the tissue development in varying degrees of proliferation and differentiation. It was also found that cells grown on the 2-D surface behaved differently in cell cycle progression and did not show increased differentiation activities after growth had stopped and proliferation activities had lowered to a minimal level. The results from this study suggest that the 3-D cell organization guided by the tissue scaffold is important to tissue formation in vitro. (C) 2000 John Wiley & Sons, Inc.

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Documento generato il 21/01/21 alle ore 03:03:56