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Titolo:
Desmosomes are reduced in the mouse uterine luminal epithelium during the preimplantation period of pregnancy: A mechanism for facilitation of implantation
Autore:
Illingworth, IM; Kiszka, I; Bagley, S; Ireland, GW; Garrod, DR; Kimber, SJ;
Indirizzi:
Univ Manchester, Sch Biol Sci, Manchester M13 9PT, Lancs, England Univ Manchester Manchester Lancs England M13 9PT M13 9PT, Lancs, England
Titolo Testata:
BIOLOGY OF REPRODUCTION
fascicolo: 6, volume: 63, anno: 2000,
pagine: 1764 - 1773
SICI:
0006-3363(200012)63:6<1764:DARITM>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
EMBRYO IMPLANTATION; MESSENGER-RNA; ESTROUS-CYCLE; CELL POLARITY; RECEPTIVITY; JUNCTIONS; ADHESION; RAT; PROGESTERONE; ENDOMETRIUM;
Keywords:
female reproductive tract; uterus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Kimber, SJ Univ Manchester, Sch Biol Sci, 3 239 Stopford Bldg,Oxford Rd, Manchester M13 9PT, Lancs, England Univ Manchester 3 239 Stopford Bldg,Oxford Rd Manchester Lancs England M13 9PT
Citazione:
I.M. Illingworth et al., "Desmosomes are reduced in the mouse uterine luminal epithelium during the preimplantation period of pregnancy: A mechanism for facilitation of implantation", BIOL REPROD, 63(6), 2000, pp. 1764-1773

Abstract

Dynamic regulation of intercellular junctions is an essential aspect of many developmental, reproductive, and physiological processes. We have shown that expression of the desmosomal protein desmoplakin decreases in the luminal uterine epithelium during the preimplantation period of pregnancy in mice. By the time of implantation (between Days 4.5 and 5 of pregnancy), desmoplakin protein can barely be detected by SDS-PACE and Western blotting, and by immunocytochemistry, it is restricted to well-spaced, punctate dots atthe apicolateral junction. Using confocal XZ series and electron microscope quantitation, both the density and distribution of desmosomes along the lateral cell surfaces of luminal epithelial cells were observed to change during early pregnancy. On Day 1 of pregnancy, desmosomes were found at high density in the apicolateral junctional complex, being present here in 79% of ultrathin sections examined, whereas on Day 5, the density was much reduced (present in only 18% of ultrathin sections examined). Desmosomes were found along the lateral surfaces, at or below the level of the nucleus, in 15% of ultrathin sections examined on Day 1 of pregnancy but in only 1% on Day 5. Desmoplakin mRNA declined during the first 4-5 days of pregnancy, along with the protein, suggesting that these changes are controlled at the level of mRNA. This study shows that desmosomes are regulated during early pregnancy, and we propose that a reduction in desmosome adhesion facilitates penetration of the luminal epithelium by trophoblast cells at implantation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 11:22:40