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Titolo:
In vitro and in vivo assay systems for study of influenza virus inhibitors
Autore:
Sidwell, RW; Smee, DF;
Indirizzi:
Utah State Univ, Inst Antiviral Res, Logan, UT 84322 USA Utah State Univ Logan UT USA 84322 nst Antiviral Res, Logan, UT 84322 USA
Titolo Testata:
ANTIVIRAL RESEARCH
fascicolo: 1, volume: 48, anno: 2000,
pagine: 1 - 16
SICI:
0166-3542(200010)48:1<1:IVAIVA>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
GG167 4-GUANIDINO-2,4-DIDEOXY-2,3-DEHYDRO-N-ACETYLNEURAMINIC ACID; NEURAMINIDASE INHIBITOR; A VIRUS; IN-VITRO; AMANTADINE-RESISTANT; T-CELLS; B VIRUS; MICE; INFECTION; ZANAMIVIR;
Keywords:
influenza; zanamivir; oseltamivir;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
100
Recensione:
Indirizzi per estratti:
Indirizzo: Sidwell, RW Utah State Univ, Inst Antiviral Res, 5600 Old Main Hill, Logan, UT 84322 USA Utah State Univ 5600 Old Main Hill Logan UT USA 84322 4322 USA
Citazione:
R.W. Sidwell e D.F. Smee, "In vitro and in vivo assay systems for study of influenza virus inhibitors", ANTIVIR RES, 48(1), 2000, pp. 1-16

Abstract

Evaluation of potential influenza virus inhibitors may utilize multiple steps. First would be to determine if the viral target (e.g. influenza virus neuraminidase) being focused upon will be inhibited in the appropriate assay. Standard in vitro antiviral assays, used next in antiviral evaluations, may utilize inhibition of viral plaques, viral cytopathic effect (CPE), andviral hemagglutinin or other protein, with inhibition of viral yield used in follow-up evaluations. The CPE can be determined visually and by dye uptake, Animal models used for study of potential influenza virus inhibitors include the ferret, the laboratory mouse, and the chicken, with a variety ofparameters used to indicate the severity of the infection and its inhibition by therapy. Multiple parameters are recommended in any in vivo antiviralevaluation. The ferret and the mouse infection models have been useful in studying the development of drug resistance and the relative virulence of drug-resistant viruses. The influenza mouse model has also been of value forthe evaluation of immunomodulating effects of test compounds and for the study of the utility of antiviral drugs for use against influenza virus infections in the immunocompromised host. In considering the use of any animal model, species differences in drug pharmacology and metabolism must be taken into account. This review has described the systems which have been used most frequently by antiviral investigators, using, as examples, recent studies with the clinically approved influenza virus neuraminidase inhibitors oseltamivir and zanamivir. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 03:59:56