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Titolo:
Distribution of DNA strand breaks produced by iodine-123 and indium-111 insynthetic oligodeoxynucleotides
Autore:
Karamychev, VN; Reed, MW; Neumann, RD; Panyutin, IG;
Indirizzi:
NIH, Dept Nucl Med, Warren G Magnuson Clin Ctr, Bethesda, MD 20854 USA NIH Bethesda MD USA 20854 ren G Magnuson Clin Ctr, Bethesda, MD 20854 USA Epoch Pharmaceut, Redmond, WA USA Epoch Pharmaceut Redmond WA USAEpoch Pharmaceut, Redmond, WA USA
Titolo Testata:
ACTA ONCOLOGICA
fascicolo: 6, volume: 39, anno: 2000,
pagine: 687 - 692
SICI:
0284-186X(2000)39:6<687:DODSBP>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
I-125 LABELED OLIGONUCLEOTIDES; DECAY; TRIPLEX; DAMAGE; CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Panyutin, IG NIH, Dept Nucl Med, Warren G Magnuson Clin Ctr, 10 Ctr Dr,Room 1C401, Bethesda, MD 20854 USA NIH 10 Ctr Dr,Room 1C401 Bethesda MD USA 20854 , MD 20854 USA
Citazione:
V.N. Karamychev et al., "Distribution of DNA strand breaks produced by iodine-123 and indium-111 insynthetic oligodeoxynucleotides", ACTA ONCOL, 39(6), 2000, pp. 687-692

Abstract

Antigene radiotherapy, a procedure based on delivery of short-range Auger-electron-emitting radioisotopes to target genes via sequence-specific tripler-forming oligonucleotides, has been successfully demonstrated in vitro using the well-studied radionuclide I-125. To proceed with in vivo trials, Auger electron emitters with shorter half-lives than I-125 are required. Herewe report a study of the efficiency and distribution of sequence-specific DNA strand breaks produced by decay of I-123 and In-111. I-123 and In-111 were introduced into triplex- and duplex-forming oligodeoxyribonucleotides (ODNs) through carbohydrate linkers of various lengths. Labeling with radioiodine was performed through tributylstannylbenzamide intermediates while In-111 was attached via DTPA. The Auger-emitter-labeled ODNs were hybridized to a single-stranded DNA target, to form duplexes. After decay accumulation, the target DNA samples were assayed for strand breaks using a sequencing gel-electrophoresis technique. For the first time, we observed footprints of DNA strand breaks produced by I-123 and In-111. Most of the breaks were located within 10 nucleotides from the decay site. The yield of strand breaks per decay varies; decay of In-111 breaks DNA almost 10 times more effectively than decay of I-123. Both I-123 and In-111 are less effective in breaking DNA strands than I-125, which reflects the higher total energy of the Auger decay process of I-125.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 06:58:09