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Titolo:
Fragilysin, the enterotoxin from Bacteroides fragilis, enhances the serum antibody response to antigen co-administered by the intranasal route
Autore:
Vines, RR; Perdue, SS; Moncrief, JS; Sentz, DR; Barroso, LA; Wright, RL; Wilkins, TD;
Indirizzi:
Virginia Polytech Inst & State Univ, Dept Biochem, Fralin Biotechnol Ctr, Blacksburg, VA 24061 USA Virginia Polytech Inst & State Univ Blacksburg VAUSA 24061 VA 24061 USA
Titolo Testata:
VACCINE
fascicolo: 6, volume: 19, anno: 2000,
pagine: 655 - 660
SICI:
0264-410X(20001108)19:6<655:FTEFBF>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
ZONULA OCCLUDENS TOXIN; CHOLERA-TOXIN; EPITHELIAL-CELLS; MATRIX METALLOPROTEINASES; INTESTINAL PERMEABILITY; DIARRHEAL DISEASE; MUCOSAL IMMUNITY; TIGHT JUNCTIONS; IN-VITRO; ADJUVANT;
Keywords:
enterotoxigenic Bacteroides fragilis; fragilysin; intranasal adjuvant;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Vines, RR Virginia Polytech Inst & State Univ, Dept Biochem, Fralin Biotechnol Ctr, Blacksburg, VA 24061 USA Virginia Polytech Inst & State Univ Blacksburg VA USA 24061 USA
Citazione:
R.R. Vines et al., "Fragilysin, the enterotoxin from Bacteroides fragilis, enhances the serum antibody response to antigen co-administered by the intranasal route", VACCINE, 19(6), 2000, pp. 655-660

Abstract

Fragilysin, an extracellular zinc metalloprotease produced by enterotoxigenic strains of the anaerobic bacterium Bacteroides fragilis, disrupts the paracellular barrier by cleavage of the intercellular proteins between epithelial cells resulting in fluid secretion. Intranasal immunization of mice with fragilysin and co-administered ovalbumin (Ova) resulted in an Ova-specific serum IgG response that was over 18 000-fold higher than Ova alone, as well as detectable levels of serum IgA. Serum IgG titers were comparable with those seen when whole cholera toxin was used as the adjuvant, although the responses obtained with fragilysin showed more variability between mice,Metalloproteases to which fragilysin is structurally related were ineffective as mucosal adjuvants. Our results and similar studies with enterotoxinsthat affect the paracellular barrier suggest that alteration of mucosal permeability may Flay an important role in the mechanisms of adjuvanticity. (C) 2000 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 02:36:38