Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Concept for organ engineering: A reconstruction method of rat liver for invitro culture
Autore:
Takezawa, T; Inoue, M; Aoki, S; Sekiguchi, M; Wada, K; Anazawa, H; Hanai, N;
Indirizzi:
Kyowa Hakko Kogyo Co Ltd, Tokyo Res Labs, Tokyo 1948533, Japan Kyowa HakkoKogyo Co Ltd Tokyo Japan 1948533 Labs, Tokyo 1948533, Japan Natl Ctr Neurol & Psychiat, Natl Inst Neurosci, Dept Degenerat Neurol Dis,Tokyo 1878502, Japan Natl Ctr Neurol & Psychiat Tokyo Japan 1878502 Dis,Tokyo 1878502, Japan
Titolo Testata:
TISSUE ENGINEERING
fascicolo: 6, volume: 6, anno: 2000,
pagine: 641 - 650
SICI:
1076-3279(200012)6:6<641:CFOEAR>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
BASEMENT-MEMBRANE; TISSUE-SLICES; FIBROBLASTS; REGENERATION; INVITRO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Takezawa, T Minist Agr Forestry & Fisheries, Natl Inst Anim Ind, Dept AnimReprod, LabReprod Endocrinol, POB 5, Ibaraki, Osaka 3050901, Japan Minist Agr Forestry & Fisheries POB 5 Ibaraki Osaka Japan 3050901
Citazione:
T. Takezawa et al., "Concept for organ engineering: A reconstruction method of rat liver for invitro culture", TISSUE ENG, 6(6), 2000, pp. 641-650

Abstract

In the past decade, there have been remarkable advances in tissue engineering technology toward the goal of creating organoids in vitro from cells and cellular scaffolding. Indeed, tissue-engineered organoids such as skin and cartilage, each with comparatively simple architectures, are presently atthe clinical stage. However, conventional tissue engineering techniques have not allowed for the reconstruction of an organoid that mimics an organ of complex architecture of abundant vascular networks. We established a method for organ engineering that can remodel a rat liver into a reconstructed organoid without separating the majority of liver cells by a continuous three-step perfusion. The liver was perfused through its vascular system with a buffered balanced salt solution to cleanse blood from the organ, with a collagenase/dispase medium to deconstruct cellular scaffolds, and with a culture medium containing collagen type I to reorganize the multicellular architecture. The reconstructed organoid was then prepared by excising the perfused liver from the rat and culturing it at 37 degreesC for 2 h. Histologically healthy parenchymal hepatocytes expressing albumin were observed in the excised organoid even after culture for 3 weeks. Furthermore, a fibroblast-implanted organoid was prepared by using a culture medium containing suspended fibroblasts in the third step of the perfusion procedure, demonstrating the efficacy of heterogeneous cells for the reconstruction of an organoid. This method may be applicable to the formation of organoids from other organs, such as kidney and spleen, each of which have abundant capillaries, and therefore the method provides a novel concept for the development of lab-grown organs, i.e., organ engineering.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 13:00:20