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Titolo:
Mice deficient in hepsin, a serine protease, exhibit normal embryogenesis and unchanged hepatocyte regeneration ability
Autore:
Yu, IS; Chen, HJ; Lee, YSE; Huang, PH; Lin, SR; Tsai, TW; Lin, SW;
Indirizzi:
Natl Taiwan Univ, Coll Med, Grad Inst Med Technol, Taipei 10018, Taiwan Natl Taiwan Univ Taipei Taiwan 10018 t Med Technol, Taipei 10018, Taiwan Natl Taiwan Univ, Coll Med, Dept Pathol, Taipei 10018, Taiwan Natl Taiwan Univ Taipei Taiwan 10018 , Dept Pathol, Taipei 10018, Taiwan Natl Taiwan Univ, Coll Med, Inst Mol Med, Taipei 10018, Taiwan Natl TaiwanUniv Taipei Taiwan 10018 Inst Mol Med, Taipei 10018, Taiwan Natl Taiwan Univ, Coll Med, Lab Anim Ctr, Taipei 10018, Taiwan Natl TaiwanUniv Taipei Taiwan 10018 Lab Anim Ctr, Taipei 10018, Taiwan Natl Taiwan Univ Hosp, Natl Hlth Res Inst, Taipei, Taiwan Natl Taiwan UnivHosp Taipei Taiwan Natl Hlth Res Inst, Taipei, Taiwan Natl Taiwan Univ Hosp, Lab Med, Taipei, Taiwan Natl Taiwan Univ Hosp Taipei Taiwan Univ Hosp, Lab Med, Taipei, Taiwan
Titolo Testata:
THROMBOSIS AND HAEMOSTASIS
fascicolo: 5, volume: 84, anno: 2000,
pagine: 865 - 870
SICI:
0340-6245(200011)84:5<865:MDIHAS>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMINOGEN-ACTIVATOR PRODUCTION; TISSUE FACTOR; FACTOR-VII; METASTASIS INVIVO; TUMOR-METASTASIS; CELL-CARCINOMA; CANCER; FIBRIN; ANTICOAGULATION; COAGULATION;
Keywords:
hepsin; gene targeting and liver regeneration;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Lin, SW Natl Taiwan Univ, Coll Med, Grad Inst Med Technol, Taipei 10018, Taiwan Natl Taiwan Univ Taipei Taiwan 10018 chnol, Taipei 10018, Taiwan
Citazione:
I.S. Yu et al., "Mice deficient in hepsin, a serine protease, exhibit normal embryogenesis and unchanged hepatocyte regeneration ability", THROMB HAEM, 84(5), 2000, pp. 865-870

Abstract

Hepsin, a liver-enriched novel serine protease, has been implicated in participating with normal cell growth, embryogenesis, and blood coagulation pathway. To study its function in vivo, we have disrupted the mouse hepsin gene by homologous recombination. Targeted disruption of the hepsin gene and ablation of hepsin message were demonstrated by Southern blotting, Northernblotting and RT-PCR analysis. Homozygous hepsin -/- mice were viable, fertile, and exhibited no gross abnormalities, as judged by the size, weight and blood coagulation (PT) assays. However, the serum concentration of the bone form of alkaline phosphatase, aspartate aminotransferase, and alanine aminotransferase of the hepsin -/- mice was mildly elevated, in spire of no obvious pathological change of hepatocytes, To examine whether hepsin is involved in controlling cell growth in adult tissues, 70% hepatectomy was applied to the hepsin -/- mice. Liver regeneration proceeded normally in the hepsin -/- mice as judged by the liver mass restoration rate. These results suggest that loss of hepsin function causes no effect in cell growth and embryogenesis in vivo, which is in contradiction to the studies using in vitrocell culturing system. Moreover, gross mass regeneration of liver after damage proceeds normally in the absence of functional hepsin.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 10:46:30