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Titolo:
Regulation of GAP-43 protein and mRNA in nigrostriatal dopaminergic neurons after the partial destruction of dopaminergic terminals with intrastriatal 6-hydroxydopamine
Autore:
Iwata, S; Nomoto, M; Fukuda, T;
Indirizzi:
Kagoshima Univ, Fac Med, Dept Pharmacol, Kagoshima 8908520, Japan Kagoshima Univ Kagoshima Japan 8908520 armacol, Kagoshima 8908520, Japan
Titolo Testata:
SYNAPSE
fascicolo: 1, volume: 39, anno: 2001,
pagine: 16 - 22
SICI:
0887-4476(200101)39:1<16:ROGPAM>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADULT-RAT BRAIN; HYDROXYLASE MESSENGER-RNA; GROWTH-ASSOCIATED PROTEIN; IN-SITU HYBRIDIZATION; TYROSINE-HYDROXYLASE; INSITU HYBRIDIZATION; TRANSPORTED PROTEINS; CYTOSKELETAL PROTEIN; PARKINSONS-DISEASE; AXON REGENERATION;
Keywords:
B-50; neuromodulin; neurotoxin; retrograde degeneration; sprouting;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Iwata, S Kagoshima Univ, Fac Med, Dept Pharmacol, Sakuragaoka 8-35-1, Kagoshima 8908520, Japan Kagoshima Univ Sakuragaoka 8-35-1 Kagoshima Japan 8908520 Japan
Citazione:
S. Iwata et al., "Regulation of GAP-43 protein and mRNA in nigrostriatal dopaminergic neurons after the partial destruction of dopaminergic terminals with intrastriatal 6-hydroxydopamine", SYNAPSE, 39(1), 2001, pp. 16-22

Abstract

Changes in the level of GAP-43 and its mRNA in nigrostriatal dopaminergic neurons in an animal model of the presymptomatic period of Parkinson's disease were measured to find the characteristic features of GAP-43 in nigrostriatal dopaminergic neurons. Since the dopaminergic neurons possess a relatively large amount of GAP-43 protein and mRNA, the dopaminergic neurons mustbe endowed with specific functions related to those of GAP-43. In this study, dopaminergic axon terminals were partially destroyed by intrastriatal B-hydroxydopamine (6-OHDA). Rats were decapitated 3, 14, and 56 days following treatment. Levels of GAP-43 and tyrosine hydroxylase (TH) in the striatum were detected by immunoblotting and quantified. The number of GAP-43 mRNA-positive neurons and that of TH mRNA-positive neurons in the substantia nigra pars compacta (SNc) were detected by in situ hybridization using alkaline phosphatase (ALP)-labeled probes. Levels of GAP-43 in the striatum showed no significant alteration during the period of the experiment, although levels of TH were gradually restored. The number of GAP-43 mRNA-positive neurons as well as that of TH mRNA-positive neurons in the SNc decreased. These results suggests that dopaminergic neurons restore their axon terminals with little change in GAP-43, and that transcription and/or stability of GAP-43 mRNA in the dopaminergic neurons are susceptible to the toxin, althoughthe dopaminergic neurons can maintain the translational product in the terminals. This feature may be related with a degeneration of dopaminergic neurons in Parkinson's disease. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 22:10:00