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Titolo:
Alternative designs for construction of the class II transfer RNA tertiarycore
Autore:
Nissan, TA; Perona, JJ;
Indirizzi:
Univ Calif Santa Barbara, Dept Chem & Biochem, Santa Barbara, CA 93106 USAUniv Calif Santa Barbara Santa Barbara CA USA 93106 Barbara, CA 93106 USA Univ Calif Santa Barbara, Interdepartmental Program Biochem & Mol Biol, Santa Barbara, CA 93106 USA Univ Calif Santa Barbara Santa Barbara CA USA 93106 Barbara, CA 93106 USA
Titolo Testata:
RNA-A PUBLICATION OF THE RNA SOCIETY
fascicolo: 11, volume: 6, anno: 2000,
pagine: 1585 - 1596
SICI:
1355-8382(200011)6:11<1585:ADFCOT>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLUTAMINYL-TRANSFER-RNA; PHENYLALANINE TRANSFER-RNA; ESCHERICHIA-COLI; CRYSTAL-STRUCTURE; FUNCTIONAL CONNECTIVITY; SYNTHETASE; RECOGNITION; IDENTITY; DISCRIMINATION; NUCLEOTIDES;
Keywords:
aminoacyl-tRNA synthetases; indirect readout; RNA engineering; RNA structure; tRNA identity;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Perona, JJ Univ Calif Santa Barbara, Dept Chem & Biochem, Santa Barbara, CA 93106 USA Univ Calif Santa Barbara Santa Barbara CA USA 93106 93106 USA
Citazione:
T.A. Nissan e J.J. Perona, "Alternative designs for construction of the class II transfer RNA tertiarycore", RNA, 6(11), 2000, pp. 1585-1596

Abstract

The structural requirements for assembly of functional class II transfer RNA core regions have been examined by sequence analysis and tested by reconstruction of alternative folds into the tertiary domain of Escherichia colitRNA(2)(Gln). At least four distinct designs have been identified that permit stable folding and efficient synthetase recognition, as assessed by thermal melting profiles and glutaminylation kinetics. Although most large variable-arm tRNAs found in nature possess an enlarged D-loop, lack of this feature can be compensated for by insertion of nucleotides either 3' to the variable loop or within the short acceptor/D-stem connector region. Rare pyrimidines at nt 9 in the core region can be accommodated in the class II framework, but only if specific nucleotides are present either in the D-loop or 3' to the variable arm. Glutaminyl-tRNA synthetase requires one or two unpaired uridines 3' to the variable arm to efficiently aminoacylate several of the class II frameworks. Because there are no specific enzyme contacts in the tRNA(Gln) core region, these data suggest that tRNA discrimination byGlnRS depends in part on indirect readout of RNA sequence information.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 04:42:20