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Titolo:
The human endogenous retrovirus K Rev response element coincides with a predicted RNA folding region
Autore:
Yang, J; Bogerd, H; Le, SY; Cullen, BR;
Indirizzi:
Duke Univ, Med Ctr, Howard Hughes Med Inst, Durham, NC 27710 USA Duke Univ Durham NC USA 27710 oward Hughes Med Inst, Durham, NC 27710 USA Duke Univ, Med Ctr, Dept Genet, Durham, NC 27710 USA Duke Univ Durham NC USA 27710 , Med Ctr, Dept Genet, Durham, NC 27710 USA NCI, Frederick Canc Res Ctr, Lab Expt & Computat Biol, NIH, Frederick, MD 21702 USA NCI Frederick MD USA 21702 & Computat Biol, NIH, Frederick, MD 21702 USA
Titolo Testata:
RNA-A PUBLICATION OF THE RNA SOCIETY
fascicolo: 11, volume: 6, anno: 2000,
pagine: 1551 - 1564
SICI:
1355-8382(200011)6:11<1551:THERKR>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; NUCLEAR EXPORT; MESSENGER-RNA; THERMODYNAMIC PARAMETERS; SECONDARY STRUCTURES; ACTIVATION DOMAIN; GENE-EXPRESSION; TRANS-ACTIVATOR; TARGET SEQUENCE; IN-VIVO;
Keywords:
nuclear export; retrovirus; Rev; RNA binding; RNA structure;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Cullen, BR Duke Univ, Med Ctr, Howard Hughes Med Inst, Room 426 CARL Bldg,Res Dr, Durham, NC 27710 USA Duke Univ Room 426 CARL Bldg,Res Dr Durham NC USA 27710 710 USA
Citazione:
J. Yang et al., "The human endogenous retrovirus K Rev response element coincides with a predicted RNA folding region", RNA, 6(11), 2000, pp. 1551-1564

Abstract

Human endogenous retrovirus K (HERV-K) is the name given to an similar to 30-million-year-old family of endogenous retroviruses present at >50 copiesper haploid human genome, Previously, the HERV-K were shown to encode a nuclear RNA export factor, termed K-Rev, that is the functional equivalent ofthe H-Rev protein encoded by human immunodeficiency virus type 1, HERV-K was also shown to contain a cis-acting target element, the HERV-K Rev response element (K-RRE), that allowed the nuclear export of linked RNA transcripts in the presence of either K-Rev or H-Rev. Here, we demonstrate that the functionally defined K-RRE coincides with a statistically highly significant unusual RNA folding region and present a potential RNA secondary structure for the similar to 416-nt K-RRE, Both in vitro and in vivo assays of sequence specific RNA binding were used to map two primary binding sites for K-Rev, and one primary binding site for H-Rev, within the K-RRE. Of note, allthree binding sites map to discrete predicted RNA stem-loop subdomains within the larger K-RRE structure, Although almost the entire 416-nt K-RRE wasrequired for the activation of nuclear RNA export in cells expressing K-Rev, mutational inactivation of the binding sites for K-Rev resulted in the selective loss of the K-RRE response to K-Rev but not to H-Rev. Together, these data strongly suggest that the K-RRE, like the H-RRE, coincides with anextensive RNA secondary structure and identify specific sites within the K-RRE that can recruit either K-Rev or H-Rev to HERV-K RNA transcripts.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/10/20 alle ore 03:34:48