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Titolo:
Tissue-specific autoregulation of Drosophila suppressor of forked by alternative poly(A) site utilization leads to accumulation of the suppressor of forked protein in mitotically active cells
Autore:
Juge, F; Audibert, A; Benoit, B; Simonelig, M;
Indirizzi:
Inst Genet Humaine, F-34396 Montpellier 5, France Inst Genet Humaine Montpellier France 5 e, F-34396 Montpellier 5, France
Titolo Testata:
RNA-A PUBLICATION OF THE RNA SOCIETY
fascicolo: 11, volume: 6, anno: 2000,
pagine: 1529 - 1538
SICI:
1355-8382(200011)6:11<1529:TAODSO>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRE-MESSENGER-RNA; POLYADENYLATION FACTOR; 3'-END FORMATION; POLY(A)-BINDING PROTEIN; PROCESSING FACTOR; HOMOLOG; MELANOGASTER; CLEAVAGE; SUBUNIT; GENE;
Keywords:
autoregulation; cleavage stimulation factor; Drosophila; mitotic cells; mRNA 3 '-end processing;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Simonelig, M Inst Genet Humaine, 141 Rue Cardonille, F-34396 Montpellier 5, France Inst Genet Humaine 141 Rue Cardonille Montpellier France 5 e
Citazione:
F. Juge et al., "Tissue-specific autoregulation of Drosophila suppressor of forked by alternative poly(A) site utilization leads to accumulation of the suppressor of forked protein in mitotically active cells", RNA, 6(11), 2000, pp. 1529-1538

Abstract

The Suppressor of forked protein is the Drosophila homolog of the 77K subunit of human cleavage stimulation factor, a complex required for the first step of the mRNA 3'-end-processing reaction, We have shown previously that wild-type su(f) function is required for the accumulation of a truncated su(f) transcript polyadenylated in intron 4 of the gene. This led us to propose a model in which the Su(f) protein would negatively regulate its own accumulation by stimulating 3'-end formation of this truncated su(f) RNA, In this article, we demonstrate this model and show that su(f) autoregulation is tissue specific. The Su(f) protein accumulates at a high level in dividing tissues, but not in nondividing tissues, We show that this distribution of the Su(f) protein results from stimulation by Su(f) of the tissue-specific utilization of the su(f) intronic poly(A) site, leading to the accumulation of the truncated su(f) transcript in nondividing tissues, Utilization ofthis intronic poly(A) site is affected in a su(f) mutant and restored in the mutant with a transgene encoding wild-type Su(f) protein, These data provide an in vivo example of cell-type-specific regulation of a protein levelby poly(A) site choice, and confirm the role of Su(f) in regulation of poly(A) site utilization.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 05:22:55