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Titolo:
Changes in p53 expression in mouse fibroblasts can modify motility and extracellular matrix organization
Autore:
Alexandrova, A; Ivanov, A; Chumakov, P; Kopnin, B; Vasiliev, J;
Indirizzi:
Russian Acad Sci, Engelgart Inst Mol Biol, Moscow, Russia Russian Acad Sci Moscow Russia Engelgart Inst Mol Biol, Moscow, Russia Moscow State Univ, Belozersky Inst Physicochem Biol, Moscow, Russia MoscowState Univ Moscow Russia y Inst Physicochem Biol, Moscow, Russia Russian Canc Res Ctr, Inst Cancerogenesis, Moscow, Russia Russian Canc ResCtr Moscow Russia Inst Cancerogenesis, Moscow, Russia
Titolo Testata:
ONCOGENE
fascicolo: 50, volume: 19, anno: 2000,
pagine: 5826 - 5830
SICI:
0950-9232(20001123)19:50<5826:CIPEIM>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-SUPPRESSOR P53; WILD-TYPE P53; TRANSCRIPTIONAL REPRESSION; GENE-EXPRESSION; BINDING PROTEIN; BAX GENE; APOPTOSIS; PATHWAY; ARREST; MUTANT;
Keywords:
p53; cell shape; motility; fibronectin; focal contacts;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Chumakov, P Russian Acad Sci, Engelgart Inst Mol Biol, Moscow, Russia Russian Acad Sci Moscow Russia nst Mol Biol, Moscow, Russia
Citazione:
A. Alexandrova et al., "Changes in p53 expression in mouse fibroblasts can modify motility and extracellular matrix organization", ONCOGENE, 19(50), 2000, pp. 5826-5830

Abstract

Effects of p53 expression on cell morphology and motility were studied using the derivatives of p53-null 10(1) mouse fibroblasts with tetracycline-regulated expression of exogenous human p53, Induction of p53 expression was accompanied by significant decrease in extracellular matrix (fibronectin) and reduction of matrix fibrils, diminution of the number and size of focal contacts, decrease of cell areas, establishment of more elongated cell shape and alterations of actin cytoskeleton (actin bundles became thinner, their number and size decreased). Expression of His175 and Gln22/Ser23 p53 mutants caused no such effects. To study the influence of p53 expression on cell motility we used wound technique and videomicroscopy observation of single living cells. It was found that induction of p53 expression led to increase of lamellar activity of cell edge, However, in spite of enhanced lamellar activity p53-expressing cells migrated to shorter distance and filled thenarrow wound in longer time as compared with their p53-null counterparts. Possible mechanisms of the influence of p53 expression on cell morphology and motility are discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 10:13:48