Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
DNA strand breaks (comet assay) and embryo development effects in grass shrimp (Palaemonetes pugio) embryos after exposure to genotoxicants
Autore:
Lee, R; Kim, GB; Maruya, KA; Steinert, SA; Oshima, Y;
Indirizzi:
Univ Syst Georgia, Skidaway Inst Oceanog, Savannah, GA 31411 USA Univ SystGeorgia Savannah GA USA 31411 t Oceanog, Savannah, GA 31411 USA Comp Sci Corp, Dept Marine Sci, San Diego, CA 92110 USA Comp Sci Corp SanDiego CA USA 92110 Marine Sci, San Diego, CA 92110 USA Kyushu Univ, Dept Fisheries, Fukuoka 812, Japan Kyushu Univ Fukuoka Japan 812 u Univ, Dept Fisheries, Fukuoka 812, Japan
Titolo Testata:
MARINE ENVIRONMENTAL RESEARCH
fascicolo: 1-5, volume: 50, anno: 2000,
pagine: 553 - 557
SICI:
0141-1136(200007/12)50:1-5<553:DSB(AA>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
CALLINECTES-SAPIDUS EMBRYOS; BLUE-CRAB; DAMAGE; CELLS;
Keywords:
genotoxicants; embryo; development; DNA; shrimp; comet;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
10
Recensione:
Indirizzi per estratti:
Indirizzo: Lee, R Univ Syst Georgia, Skidaway Inst Oceanog, 10 Ocean Sci Circle, Savannah, GA 31411 USA Univ Syst Georgia 10 Ocean Sci Circle Savannah GA USA 31411 411 USA
Citazione:
R. Lee et al., "DNA strand breaks (comet assay) and embryo development effects in grass shrimp (Palaemonetes pugio) embryos after exposure to genotoxicants", MAR ENV RES, 50(1-5), 2000, pp. 553-557

Abstract

Grass shrimp embryos develop in egg sacs (stages 1-10) attached to the female for 14-20 days after which they 'hatch' from the egg sacs into a swimming zoea stage (stage 11). Until they emerge from the egg sacs, embryos depend on lipids and lipovitellin stored within the egg. The percent of embryoswhich hatch after exposure to toxicants relative to controls was the basisof an embryo development assay. Exposure of embryos to chromium(III) chloride, sodium chromate, mercuric chloride, and 2-methyI-1,2-naphthoquinone (MNQ) resulted in a reduced hatching rate. In addition to effects on embryo development, DNA strand damage tests were carried out on contaminant-exposedembryos, using the single-cell electrophoresis method often referred to ascomet assay. Development of stage 4 embryos was more affected by MNQ exposure than stage 7 embryos. The hatching rates of stages 4 and 7 embryos exposed to MNQ (172 mug/l) were 0 and 90%, respectively. DNA strand damage, measured as DNA tail moments, were 3.4 and 4.4, respectively. Thus, exposure of an early embryo stage to MNQ prevented full embryo development while development of later embryo stages was not affected. It may be that the DNA repair systems are more efficient in later embryo stages than in early stages and thus DNA damaged in the early stages affects development. (C) 2000 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 19:16:39