Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Ca2+/calmodulin mediated pathways regulate the uptake of L-DOPA in mouse neuroblastoma neuro 2A cells
Autore:
Sampaio-Maia, B; Soares-da-Silva, P;
Indirizzi:
Fac Med Porto, Inst Pharmacol & Therapeut, P-4200 Oporto, Portugal Fac MedPorto Oporto Portugal P-4200 Therapeut, P-4200 Oporto, Portugal
Titolo Testata:
LIFE SCIENCES
fascicolo: 26, volume: 67, anno: 2000,
pagine: 3209 - 3220
SICI:
0024-3205(20001117)67:26<3209:CMPRTU>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMINO-ACID-TRANSPORT; BLOOD-BRAIN-BARRIER; STRIATAL NEURONS; EPITHELIAL-CELLS; NEUROTOXICITY; L-3,4-DIHYDROXYPHENYLALANINE; THAPSIGARGIN; INHIBITION; MODULATION; GLUTAMATE;
Keywords:
L-DOPA; neuro 2A cells; Ca2+/calmodulin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Soares-da-Silva, P Fac Med Porto, Inst Pharmacol & Therapeut, P-4200 Oporto, Portugal Fac Med Porto Oporto Portugal P-4200 Oporto, Portugal
Citazione:
B. Sampaio-Maia e P. Soares-da-Silva, "Ca2+/calmodulin mediated pathways regulate the uptake of L-DOPA in mouse neuroblastoma neuro 2A cells", LIFE SCI, 67(26), 2000, pp. 3209-3220

Abstract

The present study examined the involvement of protein kinase A (PKA), protein kinase G (PKG), protein kinase C (PKC), protein tyrosine kinase (PTK) and Ca2+/calmodulin mediated pathways on the uptake of L-DOPA through the L-type amino acid transporter in Neuro 2A cells, an in vitro model of neuronal cells. Non-linear analysis of the saturation curve for L-DOPA revealed a K-m value (in muM) of 54''2 and a V-max value tin nmol mg prtotein/6 min) of 34+/-1. L-DOPA uptake was a sodium-independent process and insensitive toN-(methylamino)-isobutyric acid (MeAIB, 1 mM), but sensitive to 2-aminobicyclo(2,2,1)-heptane-2-carboxylic acid (BHC, IC50=82 muM). The Ca2+/calmodulin inhibitors calmidazolium and trifluoperazine inhibited L-DOPA (2.5 muM) uptake with IC(50)s of 33 and 105 muM, respectively. The inhibitory effect of BHC on the accumulation of L-DOPA was of the competitive type, whereas that of calmidazolium and trifluoperazine was of the non-competitive type. Modulators of PKA (cyclic AMP, forskolin, isobutylmethylxanthine and choleratoxin), PKG (cyclic GMP, zaprinast, LY 83583 and sodium nitroprusside), PKC (phorbol 12,13-dibutirate, phorbol 12-myristate 13-acetate and chelerythrine) and PTK (genistein and tyrphostin 25) failed to affect the accumulation of a non-saturating (2.5 muM) concentration of L-DOPA. It is concluded that L-DOPA uptake in Neuro 2A cells is promoted through the L-type amino acid transporter and appears to be under the control of Ca2+/calmodulin mediated pathways. (C) 2000 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 22:12:54