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Titolo:
DIFFERENTIATION OF CAMPYLOBACTER-JEJUNI AND CAMPYLOBACTER-COLI BY POLYMERASE CHAIN-REACTION
Autore:
HARMON KM; RANSOM GM; WESLEY IV;
Indirizzi:
USDA ARS,NATL ANIM DIS CTR,ENTER DIS & FOOD SAFETY RES UNIT,POB 70 AMES IA 50010 USDA ARS,NATL ANIM DIS CTR,ENTER DIS & FOOD SAFETY RES UNIT AMES IA 50010 USDA,FOOD SAFETY & INSPECT SERV,DIV MICROBIOL BELTSVILLE MD 20705
Titolo Testata:
Molecular and cellular probes
fascicolo: 3, volume: 11, anno: 1997,
pagine: 195 - 200
SICI:
0890-8508(1997)11:3<195:DOCACB>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
GUILLAIN-BARRE-SYNDROME; CHICKEN PRODUCTS; INFECTION; ASSAY;
Keywords:
CAMPYLOBACTER JEJUNI; CAMPYLOBACTER COLI; THERMOTOLERANT CAMPYLOBACTER; MULTIPLEX PCR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
K.M. Harmon et al., "DIFFERENTIATION OF CAMPYLOBACTER-JEJUNI AND CAMPYLOBACTER-COLI BY POLYMERASE CHAIN-REACTION", Molecular and cellular probes, 11(3), 1997, pp. 195-200

Abstract

A multiplex PCR assay was developed using two primer sets for the identification and differentiation of Campylobacter coil and Campylobacter jejuni. Primer Set I amplifies a 460-bp fragment present in C. coil and C. jejuni. Set II amplifies a 160-bp target unique to C. jejuni. When the assay was performed on reference strains, amplification of C. coil yielded only the 460-bp fragment. Amplification of C. jejuni generated both the 160- and 460-bp fragments. Campylobacter field strains (n=85) isolated from raw poultry were identified by PCR and by conventional biochemical methods. Species determination by the two methods agreed for 83 of the 85 isolates examined. By PCR, 23 were identified asC. coil and 62 as C. jejuni. One isolate was unidentifiable by biochemical testing. The PCR assay identified this isolate as C. coil. in addition, one strain which was identified as C. coli by biochemical testing was determined to be C. jejuni by PCR. The PCR assay offers an alternative to traditional biochemical typing methods for the identification and differentiation of C. coil and C. jejuni isolated from poultry. it is accurate, simple to perform, and can be completed within 8 h. (C) 1997 U.S. Government.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 00:59:47