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Titolo:
A plethysmometric method for gastric compliance studies in anesthetized rats
Autore:
Graca, JRV; Leal, PRL; Gondim, FDA; Rola, FH; Santos, AA;
Indirizzi:
Fed Univ Ceara, Fac Med, Dept Physiol & Pharmacol, BR-60430270 Fortaleza, Ceara, Brazil Fed Univ Ceara Fortaleza Ceara Brazil BR-60430270 BCtaleza, Ceara, Brazil
Titolo Testata:
JOURNAL OF PHARMACOLOGICAL AND TOXICOLOGICAL METHODS
fascicolo: 1, volume: 43, anno: 2000,
pagine: 25 - 30
SICI:
1056-8719(200001/02)43:1<25:APMFGC>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
INHIBITION;
Keywords:
bethanechol; gastric compliance; gastric tone; gastric volume; plethysmography; rats; yohimbine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Santos, AA Fed Univ Ceara, Fac Med, Dept Physiol & Pharmacol, CP 3157, BR-60430270 Fortaleza, Ceara, Brazil Fed Univ Ceara CP 3157 Fortaleza Ceara Brazil BR-60430270 BCzil
Citazione:
J.R.V. Graca et al., "A plethysmometric method for gastric compliance studies in anesthetized rats", J PHARM TOX, 43(1), 2000, pp. 25-30

Abstract

A new method to study gastric volume by plethysmography is presented. Twenty male Wistar rats (250-300 g) were fasted for 24 h. After anesthesia withurethane (1.2 g/kg, i.p.), a tracheostomy was performed, and cervical vessels were cannulated. A balloon catheter was introduced per os and positioned in the proximal stomach. The opposite end of the catheter was connected to a reservoir (volume = 30ml; insidediameter = 2.5cm), coupled to a plethysmometer. A standard ionic solution was used to fill the balloon (similar to3.0 mi) and the communicating vessel system. Calibration experiments (n = 5) displayed a strong (r(2) = 0.99) correlation between graded balloon-volume changes and plethysmometric recordings. Because distending pressure of the stomach remained constant, the balloon-volume recordings were taken as gastric compliance index. Gastric volume changes, mean arterial pressure, andheart rate of animals of control and experimental groups were monitored for 90 min. The data were analyzed by analysis of variance and the Student-Newman-Keuls test. In control animals (n = 5), no significant changes on gastric volume and hemodynamic values were found. Experimental animals were treated with either yohimbine (n = 5) or bethanechol (n = 5) i.v. injections. The rats received consecutive doses of yohimbine (0.5 and 1 mg/kg) or bethanechol (1.5 and 3 mug/kg), 30 min apart. Both doses of each treatment transiently induced hypotension and bradycardia (P < 0.05). Yohimbine treatment(1 mg/kg) increased gastric volume by half(P < 0.05), whereas bethanechol (3mug/kg) decreased it by 35% (P < 0.05). In summary, this work shows a suitable method to directly assess gastric compliance in anesthetized rats. (C)2000 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 10:05:26