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Titolo:
Neural tract tracing using Di-I: a review and a new method to make fast Di-I faster in human brain
Autore:
Sparks, DL; Lue, LF; Martin, TA; Rogers, J;
Indirizzi:
Roberts Ctr Alzheimers Res, Sun Hlth Res Inst, Haldeman Lab Alzheimers Dis, Sun City, AZ 85372 USA Roberts Ctr Alzheimers Res Sun City AZ USA 85372 , Sun City, AZ 85372 USA
Titolo Testata:
JOURNAL OF NEUROSCIENCE METHODS
fascicolo: 1, volume: 103, anno: 2000,
pagine: 3 - 10
SICI:
0165-0270(20001115)103:1<3:NTTUDA>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUPERIOR OLIVARY COMPLEX; INFANT-DEATH-SYNDROME; TELEOST ACTINOPTERYGIAN FISH; VENTRAL COCHLEAR NUCLEUS; CARBOCYANINE DYE; EFFERENT CONNECTIONS; STURGEON ACIPENSER; PINEAL ORGAN; NEURONS; PROJECTIONS;
Keywords:
human neuroanatomy; fast Di-I; neural tract-tracing; review;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Sparks, DL Roberts Ctr Alzheimers Res, Sun Hlth Res Inst, Haldeman Lab Alzheimers Dis, 10515 W Santa Fe Dr,POB 1278, Sun City, AZ 85372 USA Roberts Ctr Alzheimers Res 10515 W Santa Fe Dr,POB 1278 Sun City AZ USA 85372
Citazione:
D.L. Sparks et al., "Neural tract tracing using Di-I: a review and a new method to make fast Di-I faster in human brain", J NEUROSC M, 103(1), 2000, pp. 3-10

Abstract

The use of Di-I in tract-tracing is briefly reviewed and a novel delayed-fixation approach to neural tract-tracing in the postmortem human adult brain is reported. Using the new approach, fast Di-I, a highly lipophilic fluorescent dye was injected into a particular region or nucleus and labelled tracts were followed for distances of some 20-40 mm. The procedure required approximately 36 h, yielding dye penetration rates of 1.0 mm/h or more. Thiscontrasts with previous Di-I, silver impregnation, and horseradish peroxidase protocols, where the tracer penetration rate is typically 0.003 mm/h orless, and the distance traversed amounts to only a few mm even after months of incubation. The new method hinges on the simple consideration that aldehyde fixation, which is normally employed prior to administration of the marker, crosslinks membrane proteins and impedes dye diffusion. The short postmortem samples used in our protocol permit delaying fixation until after the dye has had time to penetrate, dramatically increasing the length and scope of neural circuits that can be traced. Using these methods, for example, we have confirmed the presence of an ipsilateral olivocerebellar climbing fiber projection in the human. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 21:16:54