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Titolo:
The expression of MHC class II genes in macrophages is cell cycle dependent
Autore:
Xaus, J; Comalada, M; Barrachina, M; Herrero, C; Gonalons, E; Soler, C; Lloberas, J; Celada, A;
Indirizzi:
Univ Barcelona, Fac Biol, Dept Fisiol Biol Macrofag, E-08028 Barcelona, Spain Univ Barcelona Barcelona Spain E-08028 acrofag, E-08028 Barcelona, Spain Univ Barcelona, Fdn August Pi I Sunyer, E-08028 Barcelona, Spain Univ Barcelona Barcelona Spain E-08028 Sunyer, E-08028 Barcelona, Spain
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 11, volume: 165, anno: 2000,
pagine: 6364 - 6371
SICI:
0022-1767(200012)165:11<6364:TEOMCI>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLUCOCORTICOID RECEPTOR FUNCTION; TRANSCRIPTIONAL REPRESSION; INTERFERON-GAMMA; IFN-GAMMA; TRANSLATIONAL CONTROL; TRANSACTIVATOR CIITA; HISTONE DEACETYLASE; GROWTH-FACTOR; G1 PHASE; COMPLEX;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
68
Recensione:
Indirizzi per estratti:
Indirizzo: Celada, A Univ Barcelona, Fac Biol, Dept Fisiol Biol Macrofag, Av Diagonal645,Campus Bellvitge, E-08028 Barcelona, Spain Univ Barcelona Av Diagonal 645,Campus Bellvitge Barcelona Spain E-08028
Citazione:
J. Xaus et al., "The expression of MHC class II genes in macrophages is cell cycle dependent", J IMMUNOL, 165(11), 2000, pp. 6364-6371

Abstract

Using different drugs, we stopped the cell cycle of bone marrow-derived macrophages at different points. After IFN-gamma stimulation, macrophages arrested at the G(1) phase of the cell cycle did not increase cell surface expression of the MHC class LI IA, This inhibition is specific, because, underthe same conditions, IFN-gamma induces the expression of Fc gamma receptors and the inducible NO synthase mRNA, Treatments that inhibit macrophage proliferation by blocking the cell cycle at the G(1) phase, such as adenosine, forskolin, or LPS, blocked the IFN-gamma induction of IA, Under IFN-gammatreatment, the steady-state levels of IA alpha and LAP mRNA did not increase in cells arrested at the G(1) phase and the half-life of the MHC mRNA was not modified. These data suggest that the cell cycle modulation of IFN-gamma -induced MHC II gene expression occurs at the transcriptional level. The expression of the class II transactivator mRNA induced by IFN-gamma was also blocked when macrophages were arrested at the G(1) phase of the cell cycle, suggesting that the lack of IFN-gamma response occurs at the early steps of MHC class II expression, Finally, macrophages arrested at the G(1) phase showed increased basal levels of cell surface IA due to an increase of the translational efficiency. These data show that the expression of MHC class II genes is regulated by the cell cycle.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 01:42:22