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Titolo:
Differentially expressed genes during in vitro differentiation of murine embryonic stem cells transduced with a human erythropoietin receptor cDNA
Autore:
Xia, ZB; Dai, MS; Magoulas, C; Broxmeyer, HE; Lu, L;
Indirizzi:
Indiana Univ, Sch Med, Walther Oncol Ctr, Indianapolis, IN 46202 USA Indiana Univ Indianapolis IN USA 46202 ol Ctr, Indianapolis, IN 46202 USA Indiana Univ, Sch Med, Dept Immunol Microbiol, Indianapolis, IN 46202 USA Indiana Univ Indianapolis IN USA 46202 robiol, Indianapolis, IN 46202 USA Indiana Univ, Sch Med, Dept Med Hematol Oncol, Indianapolis, IN 46202 USA Indiana Univ Indianapolis IN USA 46202 Oncol, Indianapolis, IN 46202 USA Univ London Queen Mary & Westfield Coll, Div Biomed Sci, Neurosci Sect, London E1 4NS, England Univ London Queen Mary & Westfield Coll London England E1 4NS S, England
Titolo Testata:
JOURNAL OF HEMATOTHERAPY & STEM CELL RESEARCH
fascicolo: 5, volume: 9, anno: 2000,
pagine: 651 - 658
SICI:
1525-8165(200010)9:5<651:DEGDIV>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-VITRO; ERYTHROID DEVELOPMENT; IDENTIFICATION; CYTOKINE; DISPLAY; CULTURE; PROTEIN; PROGENITORS; INVITRO; CLONING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Lu, L Indiana Univ, Sch Med, Walther Oncol Ctr, 1044 W Walnut St,R4-302, Indianapolis, IN 46202 USA Indiana Univ 1044 W Walnut St,R4-302 IndianapolisIN USA 46202 2 USA
Citazione:
Z.B. Xia et al., "Differentially expressed genes during in vitro differentiation of murine embryonic stem cells transduced with a human erythropoietin receptor cDNA", J HEMATH ST, 9(5), 2000, pp. 651-658

Abstract

Our previous study demonstrated that transduction of murine embryonic stem(ES) cells with a human erythropoietin (Epo) receptor (R) cDNA resulted inenhanced erythropoiesis in developing embryonic bodies (EBs). To address possible mechanisms of gene regulation, we compared gene expression between hEpoR cDNA-transduced ES (ES-hEpoR) cells and parental ES cells during in vitro differentiation induced by withdrawal of leukemia inhibitory factor (LIF) and cultured in the absence of Epo using differential display reverse transcriptase-polymerase chain reaction (DDRT-PCR). A total of 48 differentially expressed cDNA fragments were found; 12 were sequenced and five were confirmed by Northern blot analysis to be up- or down-regulated in ES-hEpoR cells during differentiation compared to parental ES cells. In a GenBank search of the five putatively regulated cDNA fragments, two fragments shared high sequence homology to two known genes: the Surf-6 gene and the gene forcalcyclin binding protein. Northern blot analysis demonstrated that 2.5-kband 0.3-kb transcripts of the Surf-6 gene were expressed in undifferentiated ES-hEpoR and parental ES cells at a low level, but this expression was enhanced from day 2 to 14 of differentiation after withdrawal of LIF and culture in the presence of Epo. Furthermore, the enhanced expression of these two transcripts was also noticed in EML-C1 cells, a murine multipotential hematopoietic cell line that has erythroid differentiation potential in response to Epo. In summary, our results demonstrate that Surf-6 gene expression is regulated during differentiation of hematopoietic stem/progenitor cells in response to Epo, suggesting a possible role for Surf-6 gene in erythropoiesis.

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Documento generato il 09/04/20 alle ore 13:10:35