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Titolo:
Differential expression of three different prepro-GnRH (gonadotrophin-releasing hormone) messengers in the brain of the European sea bass (Dicentrarchus labrax)
Autore:
Gonzalez-Martinez, D; Madigou, T; Zmora, N; Anglade, I; Zanuy, S; Zohar, Y; Elizur, A; Munoz-Cueto, JA; Kah, O;
Indirizzi:
UPRES A CNRS 6026, Endocrinol Mol & Reprod, F-35042 Rennes, France UPRES ACNRS 6026 Rennes France F-35042 & Reprod, F-35042 Rennes, France Univ Cadiz, Fac Ciencias Mar, Dept Biol Anim Vegetal & Ecol, Puerto Real 11510, Spain Univ Cadiz Puerto Real Spain 11510 etal & Ecol, Puerto Real 11510, Spain Natl Ctr Mariculture, IL-88112 Eilat, Israel Natl Ctr Mariculture Eilat Israel IL-88112 lture, IL-88112 Eilat, Israel CSIC, Inst Acuicultura Torre Sal, Castellon 12595, Spain CSIC Castellon Spain 12595 Acuicultura Torre Sal, Castellon 12595, Spain
Titolo Testata:
JOURNAL OF COMPARATIVE NEUROLOGY
fascicolo: 1, volume: 429, anno: 2001,
pagine: 144 - 155
SICI:
0021-9967(20010101)429:1<144:DEOTDP>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
EEL ANGUILLA-ANGUILLA; TERMINAL NERVE-CELLS; FROG RANA-RIDIBUNDA; AMINO-ACID-SEQUENCE; 2 MOLECULAR-FORMS; SPARUS-AURATA; GILTHEAD SEABREAM; MAMMALIAN GNRH; 3 FORMS; IMMUNOCYTOCHEMICAL LOCALIZATION;
Keywords:
in situ hybridization; teleost; perciform fish; reproduction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
67
Recensione:
Indirizzi per estratti:
Indirizzo: Kah, O UPRES A CNRS 6026, Endocrinol Mol & Reprod, Campus Beaulieu, F-35042 Rennes, France UPRES A CNRS 6026 Campus Beaulieu Rennes France F-35042 es, France
Citazione:
D. Gonzalez-Martinez et al., "Differential expression of three different prepro-GnRH (gonadotrophin-releasing hormone) messengers in the brain of the European sea bass (Dicentrarchus labrax)", J COMP NEUR, 429(1), 2001, pp. 144-155

Abstract

The expression sites of three prepro-gonadotrophin-releasing hormones (GnRPIs), corresponding to seabream GnRH (sbGnRH: Ser(8)-mGnRH, mammalian GnRH), salmon GnRH (sGnRH: Trp(7)Leu(8)-mGnRH), and chicken GnRH-II (cGnRH-II: His(5)Trp(7)Tyr(8)-mGnRH) forms were studied in the brain of a perciform fish, the European sea bass (Dicentrarchus labrax) by means of in situ hybridization. The riboprobes used in this study correspond to the three GnRH-associated peptide (GAP)-coding regions of the prepro-GnRH cDNAs cloned from the same species (salmon GAP: sGAP; seabream GAP: sbGAP; chicken GAP-II: cIIGAP), which show little oligonucleotide sequence identity (sGAP versus sbGAP: 42%; cIlGAP versus sbGAP: 36%; sGAP versus cIIGAP: 41%). Adjacent paraffin sections (6 mm) throughout the entire brain were treated in parallel witheach of the three anti-sense probes and the corresponding sense probes, demonstrating the high specificity of the hybridization signal. The results showed that both sGAP and sbGAP mRNAs had a broader expression in the olfactory bulbs, ventral telencephalon, and preoptic region, whereas cIIGAP mRNA expression was confined to large cells of the nucleus of the medial longitudinal fascicle. In the olfactory bulbs, both the signal intensity and the number of positive cells were higher with the sGAP probe, whereas sbGAP mRNA-expressing cells were more numerous and intensely stained in the preoptic region. Additional isolated sbGAP-positive cells were detected in the ventrolateral hypothalamus. These results demonstrate a clear overlapping of sGAP- and sbGAP-expressing cells in the forebrain of the European sea bass, incontrast to previous reports in other perciforms showing a clear segregation of these two cell populations. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/20 alle ore 21:59:06