Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Endogenous ecotropic murine leukemia viral (MuLV) envelope protein as a new autoantigen reactive with non-obese diabetic mice sera
Autore:
Choi, SE; Kim, KS; Kim, KH; Choi, UY; Kim, HM; Yoon, JW; Kang, Y;
Indirizzi:
Ajou Univ, Sch Med, Inst Med Sci, Lab Endocrinol,Paldal Gu, Suwon 442749, Kyunggi Do, South Korea Ajou Univ Suwon Kyunggi Do South Korea 442749 49, Kyunggi Do, South Korea Hallym Univ, Dept Gen Engn, Chunchon, Kangwon Do, South Korea Hallym UnivChunchon Kangwon Do South Korea hon, Kangwon Do, South Korea Ajou Univ, Sch Med, Dept Endocrinol & Metab, Suwon 442749, Kyunggi Do, South Korea Ajou Univ Suwon Kyunggi Do South Korea 442749 49, Kyunggi Do, South Korea Univ Calgary, Fac Med,Dept Microbiol & Infect Dis, Julia McFarlane Diabet Res Ctr, Lab Viral Immunopathogenesis, Calgary, AB, Canada Univ Calgary Calgary AB Canada l Immunopathogenesis, Calgary, AB, Canada
Titolo Testata:
JOURNAL OF AUTOIMMUNITY
fascicolo: 3, volume: 15, anno: 2000,
pagine: 347 - 357
SICI:
0896-8411(200011)15:3<347:EEMLV(>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
ISLET-CELL ANTIBODIES; T-CELLS; GLUTAMATE-DECARBOXYLASE; MONONUCLEAR-CELLS; MESSENGER-RNA; INSULIN; RETROVIRUS; EXPRESSION; IDDM; IDDMK(1,2)22;
Keywords:
autoantigen; envelope; IDDM; NOD; retrovirus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Kang, Y Ajou Univ, Sch Med, Inst Med Sci, Lab Endocrinol,Paldal Gu, Wonchon Dong San 5, Suwon 442749, Kyunggi Do, South Korea Ajou Univ Wonchon Dong San 5 Suwon Kyunggi Do South Korea 442749 a
Citazione:
S.E. Choi et al., "Endogenous ecotropic murine leukemia viral (MuLV) envelope protein as a new autoantigen reactive with non-obese diabetic mice sera", J AUTOIMMUN, 15(3), 2000, pp. 347-357

Abstract

The identification and characterization of autoantigens associated with autoimmune IDDM (insulin dependent diabetes mellitus) would help to elucidatethe pathogenic mechanism of this disease as well as to design antigen-based immunotherapy. Non-obese diabetic (NOD) mice have been used as the best model for studying the pathogenesis of human IDDM. To identify new autoantigens associated with IDDM, the lambda gt11-cDNA Library from MIN6N8a, NOD-derived pancreatic beta cell line, was constructed and then candidate autoantigen clones were screened with prediabetic NOD sera. Nine positive clones were selected from 2x10(5) phage plaques. The nucleotide sequencing and homology searching showed that six of the nine positive clones had part of the endogenous ecotropic murine leukemia viral (MuLV) envelope gene. Nested deletion of this envelope gene revealed that the leucine zipper region in the transmembrane domain of MuLV envelope protein was the target epitope(s) reactive with prediabetic NOD mice sera. The prevalence of MuLV envelope protein-positive antibody in NOD mice was around 46%, while the non-NOD mice strains including BALB/c, ICR, C57BL/6, and SJL/J mice did not produce this envelope protein-reactive antibody The expression of endogenous ecotropic MuLV envelope gene in NOD mouse pancreas was distinct in those with severe insulitis. However, both prediabetic and diabetic NOD mice did not show the MHC class II-restrictive cellular autoimmunity against our purified recombinant envelope protein. In this study, we showed that the endogenous ecotropicMuLV envelope protein was a new autoantigen reactive with the activated NOD humoral immune system. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 08:14:33