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Titolo:
Release of cell-free ice nuclei from Halomonas elongata expressing the icenucleation gene inaZ of Pseudomonas syringae
Autore:
Tegos, G; Vargas, C; Perysinakis, A; Koukkou, AI; Christogianni, A; Nieto, JJ; Ventosa, A; Drainas, C;
Indirizzi:
Univ Ioannina, Dept Chem, Sector Organ Chem & Biochem, GR-45110 Ioannina, Greece Univ Ioannina Ioannina Greece GR-45110 iochem, GR-45110 Ioannina, Greece Univ Sevilla, Fac Pharm, Dept Microbiol & Parasitol, Seville, Spain Univ Sevilla Seville Spain , Dept Microbiol & Parasitol, Seville, Spain
Titolo Testata:
JOURNAL OF APPLIED MICROBIOLOGY
fascicolo: 5, volume: 89, anno: 2000,
pagine: 785 - 792
SICI:
1364-5072(200011)89:5<785:ROCINF>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
MODERATELY HALOPHILIC EUBACTERIA; ESCHERICHIA-COLI; BIOTECHNOLOGICAL APPLICATIONS; ERWINIA-HERBICOLA; REPORTER GENE; PROTEIN; IDENTIFICATION; PRINCIPLES; REPETITION; PLASMID;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Drainas, C Univ Ioannina, Dept Chem, Sector Organ Chem & Biochem, GR-45110Ioannina, Greece Univ Ioannina Ioannina Greece GR-45110 45110 Ioannina, Greece
Citazione:
G. Tegos et al., "Release of cell-free ice nuclei from Halomonas elongata expressing the icenucleation gene inaZ of Pseudomonas syringae", J APPL MICR, 89(5), 2000, pp. 785-792

Abstract

Release of ice nuclei in the growth medium of recombinant Halomonas elongata cells expressing the inaZ gene of Pseudomonas syringae was studied in anattempt to produce cell-free active ice nuclei for biotechnological applications. Cell-free ice nuclei were not retained by cellulose acetate filtersof 0.2 mum pore size. Highest activity of cell-free ice nuclei was obtained when cells were grown in low salinity (0.5-5% NaCl, w/v). Freezing temperature threshold, estimated to be below - 7 degreesC indicating class C nuclei, was not affected by medium salinity. Their density, as estimated by Percoll density centrifugation, was 1.018 +/- 0.002 g ml(-1) and they were found to be free of lipids. Ice nuclei are released in the growth medium of recombinant H. elongata cells probably because of inefficient anchoring of the ice-nucleation protein aggregates in the outer membrane. The ice(+) recombinant H. elongata cells could be useful for future use as a source of active cell-free ice nucleation protein.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 01:53:27