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Titolo:
Development of an enzyme-linked immunosorbent assay for the fungicide imazalil in citrus fruits
Autore:
Watanabe, E; Watanabe, S; Ito, S; Hayashi, M; Watanabe, T; Yuasa, Y; Nakazawa, H;
Indirizzi:
Hoshi Univ, Fac Pharmaceut Sci, Dept Analyt Chem, Shinagawa Ku, Tokyo 1428501, Japan Hoshi Univ Tokyo Japan 1428501 Chem, Shinagawa Ku, Tokyo 1428501, Japan Environm Immunochem Technol Co, Satte Off, Satte, Saitama 3400193, Japan Environm Immunochem Technol Co Satte Saitama Japan 3400193 3400193, Japan Environm Immunochem Technol Co Ltd, Shizuoka Off, Kikukawa, Shizuoka 4390031, Japan Environm Immunochem Technol Co Ltd Kikukawa Shizuoka Japan 4390031 Japan Environm Immunochem Technol Co Ltd, Head Off, Minato Ku, Tokyo 1050013, Japan Environm Immunochem Technol Co Ltd Tokyo Japan 1050013 yo 1050013, Japan
Titolo Testata:
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
fascicolo: 11, volume: 48, anno: 2000,
pagine: 5124 - 5130
SICI:
0021-8561(200011)48:11<5124:DOAEIA>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; SOLID-PHASE EXTRACTION; PESTICIDE-RESIDUES; FLUORESCENCE DETECTION; AGRICULTURAL PRODUCTS; MONOCLONAL-ANTIBODIES; GAS-CHROMATOGRAPHY; THIABENDAZOLE; IMMUNOASSAY; VEGETABLES;
Keywords:
ELISA; imazalil; monoclonal antibody; citrus fruit; HPLC;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Nakazawa, H Hoshi Univ, Fac Pharmaceut Sci, Dept Analyt Chem, Shinagawa Ku, 2-4-41 Ebara, Tokyo 1428501, Japan Hoshi Univ 2-4-41 Ebara Tokyo Japan 1428501 yo 1428501, Japan
Citazione:
E. Watanabe et al., "Development of an enzyme-linked immunosorbent assay for the fungicide imazalil in citrus fruits", J AGR FOOD, 48(11), 2000, pp. 5124-5130

Abstract

Imazalil has been widely used in citrus fruits such as lemons, oranges, and grapefruits.. A competitive enzyme-linked immunosorbent assay (ELISA) wasdeveloped for the detection of residual imazalil in citrus fruits. A monoclonal antibody (MoAb) generated to the synthetic imazalil hapten (EIT-0073)-protein conjugate was used. This assay was applied to lemon, orange, and grapefruit matrices for an imazalil analysis. The acceptable residue level for lemons, oranges, and grapefruits in Japan is 5 ppm. The matrix interference was minimized by direct dilution of the sample homogenate. No further cleanup was needed. The detection limit for imazalil in these citrus fruits was 0.1 ng/mL. The recovery of each fortified citrus fruit sample was > 81.0%. The imazalil recovery measured by the proposed ELISA was compared to the recovery determined by a conventional HPLC. A good correlation was observed between the proposed ELISA and the HPLC. This proposed ELISA would be useful for monitoring for residual imazalil.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 22:55:22