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Titolo:
HOW DOES THE W434F MUTATION BLOCK CURRENT IN SHAKER POTASSIUM CHANNELS
Autore:
YANG YS; YAN YY; SIGWORTH FJ;
Indirizzi:
YALE UNIV,SCH MED,DEPT CELLULAR & MOL PHYSIOL,333 CEDAR ST NEW HAVEN CT 06520 YALE UNIV,SCH MED,DEPT CELLULAR & MOL PHYSIOL NEW HAVEN CT 06520
Titolo Testata:
The Journal of general physiology
fascicolo: 6, volume: 109, anno: 1997,
pagine: 779 - 789
SICI:
0022-1295(1997)109:6<779:HDTWMB>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
C-TYPE INACTIVATION; K+-CHANNEL; COOPERATIVE INTERACTIONS; GATING CURRENTS; RAT-BRAIN; PORE; REGION; STOICHIOMETRY; MECHANISMS; EXPRESSION;
Keywords:
ION CHANNEL GATING; INACTIVATION; MUTATION; PATCH CLAMP; POTASSIUM CHANNELS, PHYSIOLOGY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
Y.S. Yang et al., "HOW DOES THE W434F MUTATION BLOCK CURRENT IN SHAKER POTASSIUM CHANNELS", The Journal of general physiology, 109(6), 1997, pp. 779-789

Abstract

The mutation W434F produces an apparently complete block of potassiumcurrent in Shaker channels expressed in Xenopus oocytes. Tandem tetrameric constructs containing one or two subunits with this mutation showed rapid inactivation, although the NH2-terminal inactivation domain was absent from these constructs. The inactivation showed a selective dependence on external cations and was slowed by external TEA; these properties are characteristic of C-type inactivation. Inactivation was,however, incompletely relieved by hyperpolarization, suggesting the presence of a voltage-independent component. The hybrid channels had near-normal conductance and ion selectivity. Single-channel recordings from patches containing many W434F channels showed occasional channel openings, consistent with open probabilities of 10(-5) or less. We conclude that the W434F mutation produces a channel that is predominantly found in an inactivated state.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 12:41:25